SuRe sequences

The table below carries links to fasta and GenBank flat files for the SuRe constructs carried in SuRe R1, R2 and R2-HACK strains. For more information on the SuRe system see Luo et al 2025 and our SuRe-CR and SuRe-HACK pages.

We have additional information on the adaptors below in a downloadable form: sure_adaptors.xlsx or sure_adaptors.csv. Information includes the sequences of the gRNAs, what markers are present, some information on the homology regions present, and, for those R strains that target mini-w+, the fate of the mini-w+ after AD insertion.

SuRe constructfasta fileGenbank flat file
R1 - AD1(Bxb1)1sure.ad1bxb1_1.fasure.ad1bxb1_1.gbk
R1 - AD1(Bxb1)2sure.ad1bxb1_2.fasure.ad1bxb1_2.gbk
R1 - AD1(Bxb1)3sure.ad1bxb1_3.fasure.ad1bxb1_3.gbk
R1 - AD1(Bxb1)4sure.ad1bxb1_4.fasure.ad1bxb1_4.gbk
R1 - AD1(Bxb1)5sure.ad1bxb1_5.fasure.ad1bxb1_5.gbk
R1 - AD1(Bxb1)6sure.ad1bxb1_6.fasure.ad1bxb1_6.gbk
R1 - AD1(phiC31)1sure.ad1phic31_1.fasure.ad1phic31_1.gbk
R1 - AD1(TP901-1)1sure.ad1tp901-1_1.fasure.ad1tp901-1_1.gbk
R2 - AD2(Bxb1)1sure.ad2bxb1_1.fasure.ad2bxb1_1.gbk
R2 - AD2(Bxb1)2sure.ad2bxb1_2.fasure.ad2bxb1_2.gbk
R2 - AD2(Bxb1)3sure.ad2bxb1_3.fasure.ad2bxb1_3.gbk
R2 - AD2(Bxb1)4sure.ad2bxb1_4.fasure.ad2bxb1_4.gbk
R2 - AD2(Bxb1)5sure.ad2bxb1_5.fasure.ad2bxb1_5.gbk
R2 - AD2(Bxb1)6sure.ad2bxb1_6.fasure.ad2bxb1_6.gbk
R2 - AD2(phiC31)1sure.ad2phic31_1.fasure.ad2phic31_1.gbk
R2 - AD2(TP901-1)1sure.ad2tp901-1_1.fasure.ad2tp901-1_1.gbk
R2 GAL4 HACK - AD2 p65.ADhack-gal4_p65adzp_ad2.fahack-gal4_p65adzp_ad2.gbk
R2 GAL4 HACK - AD2 QF.DBDhack-gal4_zpqfdbd_ad2.fahack-gal4_zpqfdbd_ad2.gbk