LOCUS SuRe.AD1(PhiC31) 12488 bp DNA circular SYN 26-APR-2025 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12488) AUTHORS Junjie Luo TITLE Direct Submission JOURNAL Exported Apr 26, 2025 from SnapGene 8.0.3 https://www.snapgene.com FEATURES Location/Qualifiers source 1..12488 /mol_type="other DNA" /note="color: #ffffff" /organism="synthetic DNA construct" misc_feature 43..50 /label=SbfI /note="color: #a6acb3" promoter join(71..75,76..86,92..102,107..117,150..157,158..163, 191..276) /label=3xP3 hsp70promoter /note="Direct regulation of rhodopsin 1 byPax-6/eyeless in Drosophila: evidencefor a conserved functionin photoreceptors: TAATYNRATTA" /note="This forward directional feature has 7 segments: 1: 71 .. 75 / #ffffff 2: 76 .. 86 / #cc99ff / Pax6(ey) binding site 3: 92 .. 102 / #cc99ff / Pax6(ey) binding site 4: 107 .. 117 / #cc99ff / Pax6(ey) binding site 5: 150 .. 157 / #ffffff / HSP70 core 6: 158 .. 163 / #00ff00 / TATA box 7: 191 .. 276 / #ffffff / transcription starting" regulatory 290..299 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /note="color: #a6acb3" /regulatory_class="other" CDS join(296..298,299..301,302..1015) /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 296 .. 298 / #05fd14 2: 299 .. 301 / #05fd14 / 1a 3: 302 .. 1015 / #05fd14" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" polyA_signal 1156..1277 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" misc_feature 1278..1285 /label=SbfI /note="color: #a6acb3" promoter 1320..1740 /label=dU6-3 promoter /note="RNA polymerase III promoter for Drosophila U6-3 snRNA (Port et al., 2014)" /note="color: #ffffff; direction: RIGHT" misc_feature 1743..1762 /label=gRNAu /label=attB_gRNA(core) /note="gRNAu (targets Janelia-based vector seq*) - ACCGTCGACGATGTAGGTCANGG " /note="color: #00ff00; direction: RIGHT" misc_RNA 1763..1848 /label=LgRNA scaffold /note="long gRNA, removed tttt, extended loop guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" /note="color: #00ccff; direction: RIGHT" primer_bind complement(1877..1893) /label=SK primer /note="common sequencing primer, one of multiple similar variants" /note="color: #a020f0; direction: LEFT" misc_feature complement(1910..2111) /label=PhiC31 attB /note="color: #a6acb3; direction: LEFT" protein_bind complement(2112..2147) /label=attR /bound_moiety="phage phi-C31 integrase" /note="attB site for the phi-C31 integrase (Groth et al., 2000)" /note="color: #31849b; direction: LEFT" misc_feature complement(2148..7437) /label=HA1 /note="color: #a6acb3; direction: LEFT" misc_feature complement(join(2148..2149,2153..2169)) /label=pCaryP_attP_gRNA14 /note="This reverse directional feature has 2 segments: 1: 2148 .. 2149 / #00ff00 2: 2153 .. 2169 / #00ff00" misc_feature 2148..2153 /label=SmaI/XmaI/TspMI /note="color: #a6acb3" misc_feature 2206..2211 /label=BamHI /note="color: #a6acb3" misc_feature join(2212..2219,2222..2229,2232..2239,2242..2249, 2252..2259) /label=AbsI, PspXI /note="This feature has 5 segments: 1: 2212 .. 2219 / #a6acb3 2: 2222 .. 2229 / #a6acb3 3: 2232 .. 2239 / #a6acb3 4: 2242 .. 2249 / #a6acb3 5: 2252 .. 2259 / #a6acb3" misc_feature join(2213..2218,2223..2228,2233..2238,2243..2248, 2253..2258) /label=XhoI, AvaI, SmlI, BsoBI, PaeR7I, BmeT110I /note="This feature has 5 segments: 1: 2213 .. 2218 / #a6acb3 2: 2223 .. 2228 / #a6acb3 3: 2233 .. 2238 / #a6acb3 4: 2243 .. 2248 / #a6acb3 5: 2253 .. 2258 / #a6acb3" misc_feature join(2217..2224,2227..2234,2237..2244,2247..2254) /label=EcoO109I /note="This feature has 4 segments: 1: 2217 .. 2224 / #a6acb3 2: 2227 .. 2234 / #a6acb3 3: 2237 .. 2244 / #a6acb3 4: 2247 .. 2254 / #a6acb3" misc_feature join(2218..2223,2228..2233,2238..2243,2248..2253) /label=ApaI, PspOMI, BanII, BaeGI, Bsp1286I, Bme1580I /note="This feature has 4 segments: 1: 2218 .. 2223 / #a6acb3 2: 2228 .. 2233 / #a6acb3 3: 2238 .. 2243 / #a6acb3 4: 2248 .. 2253 / #a6acb3" misc_feature 2260..2265 /label=BamHI /note="BstYI, MflI" /note="color: #a6acb3" misc_feature 2266..2271 /label=XbaI /note="color: #a6acb3" misc_feature join(2272..2275,2276..2280,2281..2285,2286..2289) /label=BfuAI,BspMI /note="This feature has 4 segments: 1: 2272 .. 2275 / #ffffff 2: 2276 .. 2280 / #a6acb3 3: 2281 .. 2285 / #a6acb3 4: 2286 .. 2289 / #ffffff" misc_feature 2272..2277 /label=AccI, HincII /note="color: #a6acb3" misc_feature 2272..2277 /label=SalI /note="color: #a6acb3" misc_feature 2275..2286 /label=DrdI /note="color: #a6acb3" misc_feature 2277..2284 /label=SbfI /note="color: #a6acb3" misc_feature 2278..2283 /label=PstI,SfcI /note="color: #a6acb3" misc_feature 2284..2289 /label=HincII /note="color: #a6acb3" misc_feature 2290..2295 /label=BamHI /note="color: #a6acb3" misc_feature complement(join(2291..2441,2442..2530,2531..2771,2772..4158, 4159..4397,4398..4568,4569..7437)) /label=mini-yellow /note="Drosophila melanogaster yellow+ transgenesismarker" /note="This reverse directional feature has 7 segments: 1: 2291 .. 2441 / #ffffff 2: 2442 .. 2530 / #ffffff 3: 2531 .. 2771 / #c0c0c0 / 3'UTR 4: 2772 .. 4158 / #ffff00 / exon 2 5: 4159 .. 4397 / #ffff00 / exon 1 6: 4398 .. 4568 / #c0c0c0 / 5'UTR 7: 4569 .. 7437 / #ffffff / promoter" misc_feature 2950..2957 /label=SbfI /note="color: #a6acb3" CDS complement(3697..3708) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /note="color: #cc99b2 Cleavage site after base 3696" /translation="IEGR" misc_feature 7432..7437 /label=HA1 cutting /note="color: #a6acb3" misc_feature complement(7438..9103) /label=HA2 /note="color: #a6acb3; direction: LEFT" misc_feature 7485..7496 /label=SapI cutting /note="The SapI works at 37C. It is better than BspQI which works at 50C." /note="color: #a6acb3" misc_feature 8359..8366 /label=FseI /note="color: #a6acb3" misc_feature 8359 /label=attP40 /label=nonstandard type: transposable_element_insertion_site /note="/name=FBti0114379" /note="/score=0" /note="color: #ff0000" misc_feature join(8366..8396,8397..8418,8419..8951) /label=P element 5' end /note="This feature has 3 segments: 1: 8366 .. 8396 / #ffe4c4 / inverted repeat 2: 8397 .. 8418 / #ffe4c4 3: 8419 .. 8951 / #ffe4c4" protein_bind complement(join(9078..9103,9104..9137)) /label=attL /bound_moiety="phage phi-C31 integrase" /note="attB site for the phi-C31 integrase (Groth et al., 2000)" /note="This reverse directional feature has 2 segments: 1: 9078 .. 9103 / #ff00ff / attP 2: 9104 .. 9137 / #31849b / attB" misc_feature complement(join(9139..9141,9142..9144,10676..10692)) /label=gRNAu target with AD1(PhiC31)1 insertion /label=attB_SgrDI_gRNA /note="This reverse directional feature has 3 segments: 1: 9139 .. 9141 / #00ff00 2: 9142 .. 9144 / #00ff00 3: 10676 .. 10692 / #00ff00" misc_feature join(9158..10198,10220..10269,10277..10662) /label=[AD1_P^{PhiC31}] /note="This forward directional feature has 3 segments: 1: 9158 .. 10198 / #ff0000 / 3xP3-mCherry 2: 10220 .. 10269 / #ff00ff / attP(PhiC31) 3: 10277 .. 10662 / #31849b / Insulator" polyA_signal 9158..9279 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" CDS complement(9286..9996) /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /note="color: #ff0000" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" misc_feature 9639..9646 /label=SbfI /note="color: #a6acb3" promoter complement(join(10007..10092,10120..10125,10126..10131)) /label=hsp70 mini /note="Drosophila melanogaster hsp70Bb promoter" /note="This reverse directional feature has 3 segments: 1: 10007 .. 10092 / #ffffff / align to 3xP3 2: 10120 .. 10125 / #cc99ff / TATA box 3: 10126 .. 10131 / #ffffff" promoter complement(join(10152..10162,10167..10177,10183..10193, 10194..10198)) /label=3xP3 /note="Direct regulation of rhodopsin 1 byPax-6/eyeless in Drosophila: evidencefor a conserved functionin photoreceptors: TAATYNRATTA" /note="This reverse directional feature has 4 segments: 1: 10152 .. 10162 / #cc99ff / Pax6(ey) binding site 2: 10167 .. 10177 / #cc99ff / Pax6(ey) binding site 3: 10183 .. 10193 / #cc99ff / Pax6(ey) binding site 4: 10194 .. 10198 / #ffffff" misc_feature complement(10220..10269) /label=attP /note="color: #ff00ff; direction: LEFT" protein_bind 10277..10662 /label=gypsy insulator /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2" /note="chromatin insulator from Drosophila" /note="color: #31849b" rep_origin complement(10755..11343) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(join(11514..12305,12306..12374)) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 11514 .. 12305 / #ccffcc 2: 12306 .. 12374 / #ccffcc / signal sequence Cleavage site after base 12305" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(12375..12479) /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: LEFT" ORIGIN 1 ttaattaact cgcgttaaga tacattgatg agtttggaca aacctgcagg ccctttcgtc 61 ttcaagaatt ggatctaatt caattagaga ctaattcaat tagagctaat tcaattagga 121 tccaagctta tcgatttcga accctcgacc gccggagtat aaatagaggc gcttcgtcta 181 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca 241 aataaacaag cgcagctgaa caagctaaac aatcggctcg aagccggtcg ccaccatggt 301 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 361 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 421 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 481 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 541 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 601 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 661 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 721 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 781 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 841 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 901 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 961 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg 1021 cctaagcgat cgctcaggcc gcgactctag atcataatca gccataccac atttgtagag 1081 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 1141 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 1201 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 1261 ctcatcaatg tatcttacct gcaggagtaa actcgtcgac ggtatcgata agcgctagct 1321 tttttgctca cctgtgattg ctcctactca aatacaaaaa catcaaattt tctgtcaata 1381 aagcatattt atttatattt attttacagg aaagaattcc ttttaaagtg tattttaacc 1441 tataatgaaa aacgattaaa aaaaatacat aaaataattc gaaaattttt gaatagccca 1501 ggttgataaa aattcatttc atacgtttta taacttatgc ccctaagtat tttttgacca 1561 tagtgtttca attctacatt aattttacag agtagaatga aacgccacct actcagccaa 1621 gaggcgaaaa ggttagctcg ccaagcagag agggcgccag tgctcactac tttttataat 1681 tctcaacttc tttttccaga ctcagttcgt atatatagac ctattttcaa tttaacgtcg 1741 gtaccgtcga cgatgtaggt cagtcttaga gctatgctgg aaacagcata gcaagttaag 1801 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttatcgaa 1861 ttcctgcagc ccgggggatc cactagttct agagcggcgc gcccatatgt tcggcttgtc 1921 gacatgcccg ccgtgaccgt cgagaacccg ctgacgctgc cccgcgtatc cgcacccgcc 1981 gacgccgtcg cacgtcccgt gctcaccgtg accaccgcgc ccagcggttt cgagggcgag 2041 ggcttcccgg tgcgccgcgc gttcgccggg atcaactacc gccacctcga cccgttcatc 2101 atgatggacc agatgggtga ggtggagtac gcgcccgggg agcccaaccc ggggcatccg 2161 ctgagggttg ccgccggggc ttcggtgtgt ccgtcagtac tagggggatc ccctcgaggg 2221 ccctcgaggg ccctcgaggg ccctcgaggg ccctcgaggg gatcctctag agtcgacctg 2281 caggtcaacg gatcctaccc taccaccaac aaagtctttt tgtttgcccc gtctgaagtc 2341 tggctccgat caaaagcaaa aatttcaatt acagtcgaat ctcaagcgac caggcgatct 2401 caaattgagc gattaagttg aactttccct gcacccaaac aagatcttgt gcatagcttt 2461 atcaacctac tttcttgcct ggtagtagta ctccagaaaa tgtacagttg ttgatatatt 2521 tagaatttat gcatacttac attttttccg ctttttccgc tcaagaaaat tgcgtaaact 2581 cttaaccttc aaaaaaaagt tgatttattg ttattttttg cttaacataa ctagataaag 2641 tattgatttg ccacttgctc atacgtcatg tggttttttt aaccgctgtt gccctatgtt 2701 gttgtcttta aatattcttt acatcaatcg agtgtgtgag aatataccca agtaccgtgt 2761 gtaggattat gttaaccttg atgctgatga tgccaccacc cagattggcg ggcattcaca 2821 taagttttta acccaccatc ctggccaccc ggttgggcgg gttgatgggt gggaaataga 2881 tggggaccac ttgtctcgta atttatgccg ttgtgctggt tgaaaatata ggcctttggc 2941 acctccactc ctgcaggacc cacagaattt gtagagacac taatactgga gactacattg 3001 cctgaattgg cgggcaaata agtgcgactt ggaggaggcg gcgaggaagc ccagctggtc 3061 tgaggtttct gtggcaagac aggacgatat tgtttcgtat ataacggtgg acccattggc 3121 aaaacggctt gttttggtat tgaaacggta tttggcggcc cataggcgtt attcctcaaa 3181 tcacacacag tattctcaat taaagtggcc aagggagccg tgtaaattcg gaaattagta 3241 tctgaataat ccaagtcaga cagcaagaaa acgggcatcc tatcggatag aacccaaacg 3301 tttttgttct catcaatttt cacatcggcc ggaaaaacta agccaacgtc atcgcgatcc 3361 acaatgccat gaaattgcgg tgagtacggc attgatgagt gccagcaacc cactgcattt 3421 tgatctatta aattgaacag ctcaattcca tcatcgctca tcacacgtga agtggtatgg 3481 gagtttggac cccgttcatc taaggcaaca aagtcatgat agctatcttc cgtcctggtt 3541 tcatccctca aaatcctcgt ggatacggca aattgtcgat gacttgctaa cggactaaag 3601 tacagggtac gataaccatc cgatcgaatg ggcgaaaggg acataccaaa tataccctcc 3661 tcgccccatt ggaagttaat accagcgaca ttgaaatcgc ccctcaatgg atcggggaaa 3721 aaatacgaat gtgccgagaa tctccaggac ttgttcagtt cccaggagta agcaatcaag 3781 ccgtatccca attcatcggc aaaataggca tatgcatcat cgcaattttt gcctatatcc 3841 acggcaatgt tagctatgaa agtatttgga tttgtgtcca cgccaggtag ctcgtatctc 3901 cgaattcgcg tatccgtggt caagtcaaag acatttaccg cataggggca cggattagtg 3961 gtggtattgc cgatgcccac ggttccagtg tccaaaaccc acagccgacc acactcatcc 4021 actttaatgc ggtaggcagt ggtaatactg ttggcgcaat ctccagctgt atttgagcgc 4081 caatctggat acggaattag ctccggtgaa cccgtcaaac tgcggtccat gtttatatag 4141 gtcagagtgg ccggaatccc atcacgccag cggggaacag tgacgaataa ccgattgcca 4201 aagtgttcga ctccaacagg tagagcattt tgcggaatat aatctccact agccagagct 4261 tggtccttta gtcgggtatt cgggaaagca aagtccagct ggctccaact atatcgctcc 4321 tgaagtttgt aagcagccca agacggcgtc accaaggtga tcagggtcac aaggatccac 4381 cctttgtcct ggaacattgc acttagctct aagctgacaa tcactcttaa cacttaagcc 4441 aaagtgtgtt ggcaataaaa aaaaatatat ttgtttatat atgttcagct atagggggtt 4501 cttctctagc cttcggctgt gtgatatttt tttaatcctc ttctgtggac cgtggcgcgg 4561 taacgactgg tggccataat atgtcggccg cgttttatat gaaggttttt ttctccgaag 4621 acgaagacag gccaatgaaa atgaaaacga aggcgcgcgc caacttcggt ggttttagtg 4681 attcgggtgg ttcagtgttc gggtaatcag gtggcttatg ctgttcccat agatcggcca 4741 actttgcgtt ttgtcttcca tgattgattt tcacgcatgt ttaataaata cactcgaatc 4801 ccccaattta atgagtcaga catcaccttt cgctgggttt ggtatgattt ttggccttca 4861 tcgacattta aaactgcgct gatttgtgcc gaaatacctt tcggtaccct aaaagattaa 4921 gacctatttt aaagttgtga ctactattta tttaatttta gggactttat ttgacgatct 4981 aatttcaaac aagtaaattg taaggccagg aggctcgtgc atagaatgca ttgctctcca 5041 aatttctgaa aatctgaaag cttaatacat gacagttgtg ttctgagaac agtgttcatc 5101 tttatcggcg actgcaaaaa atgttctaat atgatttttt agttcaattc attaaaaaaa 5161 tcaaatcaat attatatgaa atacatgcag acaaaaatcc agaaatatca aataaatcgt 5221 tattataacg tgtactatgt agagtattta aagcaggtgt ttccttgtat actattaaat 5281 tggaactcgt gctcgtttca attgcttagg attggatttc gattgggcgt caccgatgat 5341 tcacaattca ctgagggtca aatatttggt ttccgctagt tattggcagg tgattttgag 5401 catacatata cataaatata attgagtgtt acataaaatt tcaaaaggga cgaaagcgct 5461 tttaggtaat tccactagct gttatttaaa atattgtggt ccctgaaaat caactggatg 5521 ttgggggtgc cctaagtggg taatgctcta atgaccatcg ccgcaagtaa atgttaaaaa 5581 ttcgaaccga aagcgatcgc atatatctct tggcccaaat gcacctgctt gctaaagaaa 5641 aaattgcaaa acttaagtaa agtaatacaa ccagccacac gcataataaa catgcaaatg 5701 ggtaaaaata cgttttacga cctccgcaga taaagtggtt tgcattaatc ctcaataatc 5761 tacactttaa ataaggaaaa cctctcaaaa tattaaaata caaattaaag aatgccgttt 5821 aaatttaatc ttatttcttc ggatgtctaa aaacaatttc aaagctaatg taaccattac 5881 tgctattgaa tttgtgtagt ttttggactc tatatcattt atgcaatgct attttttctt 5941 agaagtggtt caacttgatc ccttgcccgc attctaaatc attgcaccca cagaaaaata 6001 aacaaacacg gtgtggcaat cgaaaaatat gttgcacaaa attaccggca atgctgctcg 6061 aatttattgc gatcggcagg cttattaaaa gacacacttg aaataaccag tacagtttta 6121 ttaattaatt gtgtgtgttg tgtaatgaaa tggtgtattt actaaaaacg caactaatga 6181 tgcgatcgct gcagccgttg aacctataat ttttttcgaa ttgacttgtt ttcaagtttt 6241 tgcttaattg tgtgggggga tgctgcgttt gtctgtttgg ttactgtttt tctttttcgc 6301 ttttcagagc ttgcggcaat gatttatacg cacttttccc aataattgaa acaagttttc 6361 tgccccagca caaaacaaaa caacatcaag cggagcgcgc taagtggtca aaaaaagctt 6421 ccacaaatca gatctctttg gcataattga ctaacgctta tgcaaccgac cggcaacatt 6481 cgacattatt tacacacagg atcacttcaa cttgggcgga aagcaaagcc gaataccgca 6541 tgacattgcc cgacccgtgt ttcatgtttt attttcctta ttccccactt atggcagcct 6601 ggacattatc tccctccctc ccctcccccc tcctacacta atttggaaat ccatcgtgac 6661 tgcaaaacga aaaacccgtt gctcaacttc tctaacataa gcggtacaca gcattttccg 6721 ttttgtgtta tatatttttg tgtttttttt tttcaagttc aatgtccatg gccctgaccg 6781 ccgggtcccc tcctttcgag ctgggattat ttggcatggc caaattcgga aggccgcgat 6841 cacttcccgc ttacacacaa ttgcacatgt ccccatgaca tgcactgcgc tcaaactatt 6901 agtacccctt agtaacttaa gccaaattaa aaataagttc attaatataa ataaaatagt 6961 caatttccac tgtcccgatt gaatagagaa actttatttg taaattttaa atgtcgccaa 7021 ggaactcgct agatatacta aaccaaaacc gattgtagct cccgaaatcg caaccataag 7081 tggaataatt agatgccaac aaaggctttg tttgcttact gaataaaggt tgtcgtaatt 7141 tgatttctgt gtaatcttgc acgaaacgga atcattacct cagacataat ttgcctgcaa 7201 ttgcggtggt gcacttgtct cgtgcaagcg aaaaaatcag tgctcgaaaa agcttagtgg 7261 atatactcgt acatatacta tgaatcgttt agaggggtag gtaaatcagc gggctgcgtt 7321 cgaaatttat gagtgactct gcgacgtatt tatcttaagc catcgtcagt tgcatatttt 7381 atggctattt taaaccactc aatgtggtaa tcgggcgata atcatttaat agtcgacatg 7441 gtcttcatgt aggtcctcgt caccgtcgat gtctccagct gttggctctt ctcctcctcc 7501 agcacctgct ctctcgagga tttctgctca tgcgcacgac gccggcgaac agctgggact 7561 ggaggcacgt catcacctcc ggctgctcct gctccgcccc cgccggcatc tatggttgtt 7621 ggatccatgt gcttggatcc tggtcctcca ctatcagcca taatagcatt gatttcgcta 7681 atagaccctc gtcaagccaa attgtcatcg gtcacgagtt tttcgcagcc ccaaaaaacc 7741 agcctttaat tcgtgtcctt ttctcctcgc tccaccaatt ttctctccgt tttcggcttt 7801 ccaccaaatg cagaattgat ttttctcacg caacgcacac acactcacac acacacacac 7861 ggtgactttt caccgaattt tctctttgaa caacccttga atggccttca gtctttaatt 7921 tttcggccca cgtaattcaa ccttaaattt ttcccaaatg caaatacaca cgcttcttgg 7981 ctattttatt atattaaaca cttgaagctt tgggcttttg gggattttat tgcgcgacac 8041 acaatgccaa ccggagatta tctcacgcag ttaggtaagg aaattagtat ttcgctcgca 8101 attcttctcg cagtcccaca aaaaaagatg cgagaattcg ggggctttcg gtgtttatta 8161 cttttatttc agttttgcct gctgaacata gccggctata tcacattatt acccggcgac 8221 tttgtcgctc cgcgcttcca atccgctgtc gtagaaagcc ggttcgatac taaacccaaa 8281 cccactgccg ttcaatgtgc tacgccttgt ccagtccaaa aagtcgcgag agaagagctg 8341 tatagtaatc gtagtaaagg ccggccatga tgaaataaca taaggtggtc ccgtcgatag 8401 ccgaagctta ccgaagtata cacttaaatt cagtgcacgt ttgcttgttg agaggaaagg 8461 ttgtgtgcgg acgaattttt ttttgaaaac attaaccctt acgtggaata aaaaaaaatg 8521 aaatattgca aattttgctg caaagctgtg actggagtaa aattaattca cgtgccgaag 8581 tgtgctatta agagaaaatt gtgggagcag agccttgggt gcagccttgg tgaaaactcc 8641 caaatttgtg atacccactt taatgattcg cagtggaagg ctgcacctgc aaaaggtcag 8701 acatttaaaa ggaggcgact caacgcagat gccgtaccta gtaaagtgat agagcctgaa 8761 ccagaaaaga taaaagaagg ctataccagt gggagtacac aaacagagta agtttgaata 8821 gtaaaaaaaa tcatttatgt aaacaataac gtgactgtgc gttaggtcct gttcattgtt 8881 taatgaaaat aagagcttga gggaaaaaat tcgtactttg gagtacgaaa tgcgtcgttt 8941 agagcagcag ccgaattggc ggccgccggc ggccgccaat tcgagctcgc ccctagtcgc 9001 gctcgcgcga ctgacggtcg taagcacccg cgtacgtgtc caccccggtc acaacccctt 9061 gtgtcatgtc ggcgacccta cgcccccaac tgagagaact caagggcacg ccctggcacc 9121 cgcaccgcgg cttcgagacc gtgactcgag acctaggtaa gatacattga tgagtttgga 9181 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt 9241 gctttatttg taaccattat aagctgcaat aaacaagtta ccggtttact tgtacagctc 9301 gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgatggtgta 9361 gtcctcgttg tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg 9421 cacgggcttc ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt 9481 cagcttcagc ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc 9541 ggaggaggcc tcccagccca tggttttctt ctgcattacg gggccgtcgg aggggaagtt 9601 ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt 9661 cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg 9721 gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc 9781 gtacatgaac tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac 9841 cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc 9901 gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga actccttgat 9961 gatggccatg ttatcctcct cgcccttgct caccatgttg acgcgtccga ttgtttagct 10021 tgttcagctg cgcttgttta tttgcttagc tttcgcttag cgacgtgttc actttgcttg 10081 tttgaattga attgtcgctc cgtagacgaa gcgcctctat ttatactccg gcggccgcta 10141 gagtctccgc ttaattgaat tagctctaat tgaattagtc tctaattgaa ttagatccgg 10201 gtcacggaag ttagcatgcc tacgccccca actgagagaa ctcaaaggtt accccagttg 10261 gggcactacg agctcgaatt gatcggctaa atggtatggc aagaaaaggt atgcaatata 10321 ataatctttt attgggtatg caacgaaaat ttgtttcgtc aacgtatgca atattcttta 10381 ttaaaagagg gtatgcaatg tattttatta aaaacgggta tgcaatataa taatctttta 10441 ttgggtatgc aacgaaaatt tgtttcgtca aagtatgcaa tattttttat taaaagaggg 10501 tatgcaatgt attttattaa aaacgggtat gcaataaaaa attatttggt ttctctaaaa 10561 agtatgcagc acttattttt tgataaggta tgcaacaaaa ttttactttg ccgaaaatat 10621 gcaatgtttt tgcgaataaa ttcaacgcac acttattacg tgggcccaca cgtggcctac 10681 atcgtcgacg gtaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 10741 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 10801 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 10861 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 10921 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 10981 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 11041 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 11101 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 11161 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 11221 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 11281 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 11341 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 11401 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 11461 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 11521 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 11581 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 11641 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 11701 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 11761 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 11821 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 11881 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 11941 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 12001 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 12061 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 12121 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 12181 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 12241 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 12301 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 12361 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 12421 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 12481 acatttcc //