The Super Recombinator Cas9 with Serine Recombinase (SuRe-CR) system was designed by Junjie Luo and Mark Schnitzer to recombine insertions in the same attP landing site onto the same chromosome with simple genetic crosses utilizing one of three serine recombinases (phiC31, Bxb1 or TP901-1). Two to many insertions can be put in the same landing site with successive rounds of recombination.

Briefly, Recombinator 1 (R1) and Recombinator 2 (R2) strains carrying Cas9 are used to put adaptors (AD1, carrying an attP site, and AD2, carrying an attB site) upstream (using gRNAu) and downstream (using gRNAd) of the transgenes in question. A serine recombinase (phiC31, Bxb1 or TP901-1) is then used to recombine the two together via the attP/attB sites. See Luo et al 2025 for details.

We have additional information on the adaptors donated to the BDSC in this downloadable Excel file, sure_adaptor.xlsx, including useful information regarding the fate of the mini-w+ after AD2 insertion, the vectors targeted by gRNAu, the sequences of the gRNAs, and what markers are present. 

R1 and R2 transgene sequences can be found on the SuRe sequences page.

Schnitzer lab has also created lines for HACKing an adaptor plus coding sequences for a split GAL4 component into an existing GAL4 insertion in one step. See the SuRe-HACK page for more information.