The Super Recombinator (SuRe) system was designed by Junjie Luo and Mark Schnitzer to recombine insertions in the same attP landing site onto the same chromosome with simple genetic crosses. Two to many insertions can be put in the same landing site with successive rounds of recombination. See Luo et al., 2025 and our SuRe-CR page for more information.

To combine a GAL4.DBD and a p65.AD into the same chromosome, they must be in the same landing site. In the Janelia split-GAL4 collection, however, most GAL4.DBD insertions are in attP2 and most p65.AD insertions are in attP40. One must therefore first 'move' one of the splits to the other site before SuRe will work. To facilitate this, Schnitzer lab has created lines for HACKing an adaptor plus coding sequences for p65.AD or QF.DBD into an existing GAL4 insertion in attP2 in one step. We carry GAL4 cognates in attP2 for most of the Janelia "Rubin" p65.AD lines in attP40 and VK00027. You can find GAL4 cognates in attP2 for some "VT" p65.AD lines at the Vienna Drosophila Resource Center.

See our GAL4 and split-GAL4 pages for lists of existing lines carrying GAL4, GAL4.DBD, p65.AD and other split-GAL4 components.

Sequence files for SuRe components can be found on our SuRe sequences page.