LOCUS SuRe.AD2(TP901-1 9506 bp DNA circular SYN 26-APR-2025 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9506) AUTHORS Junjie Luo TITLE Direct Submission JOURNAL Exported Apr 26, 2025 from SnapGene 8.0.3 https://www.snapgene.com FEATURES Location/Qualifiers source 1..9506 /mol_type="other DNA" /note="color: #ffffff" /organism="synthetic DNA construct" source 136..145 /chromosome="2R" /mol_type="genomic DNA" /note="/genotype=y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2]bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]" /note="color: #ffffff" /db_xref="taxon:7227" /organism="Drosophila melanogaster" source 166..175 /chromosome="2R" /mol_type="genomic DNA" /note="/genotype=y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2]bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]" /note="color: #ffffff" /db_xref="taxon:7227" /organism="Drosophila melanogaster" source 196..205 /chromosome="2R" /mol_type="genomic DNA" /note="/genotype=y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2]bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]" /note="color: #ffffff" /db_xref="taxon:7227" /organism="Drosophila melanogaster" source 6274..6283 /chromosome="2R" /mol_type="genomic DNA" /note="/genotype=y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2]bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]" /note="color: #ffffff" /db_xref="taxon:7227" /organism="Drosophila melanogaster" source 6304..6313 /chromosome="2R" /mol_type="genomic DNA" /note="/genotype=y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2]bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]" /note="color: #ffffff" /db_xref="taxon:7227" /organism="Drosophila melanogaster" source 6334..6343 /chromosome="2R" /mol_type="genomic DNA" /note="/genotype=y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2]bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]" /note="color: #ffffff" /db_xref="taxon:7227" /organism="Drosophila melanogaster" protein_bind complement(1..70) /label=attB /bound_moiety="phage phi-C31 integrase" /note="attB site for the phi-C31 integrase (Groth et al., 2000)" /note="color: #31849b; direction: LEFT" misc_feature complement(join(72..74,75..77,78..94)) /label=gRNAu target /label=attB_R1_gRNA /note="This reverse directional feature has 3 segments: 1: 72 .. 74 / #00ff00 2: 75 .. 77 / #00ff00 3: 78 .. 94 / #00ff00" misc_feature 101..218 /label=3xTpnC41C_PCR /note="color: #a6acb3" misc_feature join(118..133,136..145,148..163,166..175,178..193,196..205) /label=3xTpnC41C /note="Expression of the Troponin C at 41C Gene in Adult Drosophila Tubular Muscles Depends upon Both Positive and Negative Regulatory Inputs Maria B. Chechenova, Sara Maes, Richard M. Cripps Published: December 7, 2015 '>https://doi.org/10.1371/journal.pone.0144615" /note="This forward directional feature has 6 segments: 1: 118 .. 133 / #a6acb3 / SM3 2: 136 .. 145 / #ff00ff / MEF2 3: 148 .. 163 / #a6acb3 / SM3 4: 166 .. 175 / #ff00ff / MEF2 5: 178 .. 193 / #a6acb3 / SM3 6: 196 .. 205 / #ff00ff / MEF2" promoter join(246..251,252..257,285..370) /label=hsp70 mini /note="Drosophila melanogaster hsp70Bb promoter" /note="This forward directional feature has 3 segments: 1: 246 .. 251 / #ffffff 2: 252 .. 257 / #cc99ff / TATA box 3: 285 .. 370 / #ffffff / align to 3xP3" regulatory 384..393 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /note="color: #a6acb3" /regulatory_class="other" CDS 390..1067 /codon_start=1 /product="rapidly maturing tetrameric variant of DsRed fluorescent protein (Bevis and Glick, 2002)" /label=DsRed-Express /note="mammalian codon-optimized" /note="color: #ff0000" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH LFL" polyA_signal 1188..1309 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" promoter 1352..1772 /label=dU6-3 promoter /note="RNA polymerase III promoter for Drosophila U6-3 snRNA (Port et al., 2014)" /note="color: #ffffff; direction: RIGHT" misc_feature join(1774..1790,1791..1793) /label=gRNAd /label=w_chiRNA_f2(core) /note="gRNAd (targets mini-w+ and makes w null) - CCGCAGTCCGATCATCGGATNGG from white eraser. sequenced" /note="This forward directional feature has 2 segments: 1: 1774 .. 1790 / #00ff00 2: 1791 .. 1793 / #00ff00" misc_RNA 1794..1879 /label=LgRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" /note="color: #00ccff; direction: RIGHT" rep_origin complement(1980..2568) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(join(2739..3530,3531..3599)) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 2739 .. 3530 / #ccffcc 2: 3531 .. 3599 / #ccffcc / signal sequence Cleavage site after base 3530" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3600..3704) /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: LEFT" misc_feature complement(join(3962..5202,6409..9286)) /label=mini-w with AD2(TP901-1)1 insertion /note="This forward directional feature has 2 segments: 1: 3962 .. 5202 / #ff0000 2: 6409 .. 9286 / #ff0000" misc_feature 4724..4743 /label=gRNAd(old version) /label=w_ApaI_gRNA /note="gRNAd ('old', targets mini-w+ and makes w hypomorph) - TGTCGGCTACTCCTTGCGTCNGG " /note="color: #00ff00; direction: RIGHT" misc_feature join(5186..5202,6409..6411,6412..6414) /label=gRNAd target with AD2(TP901-1)1 insertion /label=wChi2R2_LgRNA_0_off /note="This forward directional feature has 3 segments: 1: 5186 .. 5202 / #00ff00 2: 6409 .. 6411 / #00ff00 3: 6412 .. 6414 / #00ff00" misc_feature join(5203..5209,5210..5265,5266..5271,5272..6361, 6362..6408) /label=[AD2_B^{TP901}] /note="This forward directional feature has 5 segments: 1: 5203 .. 5209 / #ffffff 2: 5210 .. 5265 / #31849b / attB[TP901] 3: 5266 .. 5271 / #ffffff 4: 5272 .. 6361 / #00ffff / TpnC41C-CFP 5: 6362 .. 6408 / #ffffff" misc_feature complement(join(5210..5224,5225..5231,5232,5233..5237, 5238..5257,5258..5265)) /label=attB(TP901-1)Long /note="Blast by attB(TP901-1) and choose the common region" /note="This reverse directional feature has 6 segments: 1: 5210 .. 5224 / #31849b 2: 5225 .. 5231 / #31849b / identity region 3: 5232 .. 5232 / #31849b 4: 5233 .. 5237 / #31849b / core 5: 5238 .. 5257 / #31849b 6: 5258 .. 5265 / #31849b / conflict" polyA_signal 5272..5393 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" CDS complement(join(5400..6113,6114..6116,6117..6119)) /codon_start=1 /product="cyan variant of GFP" /label=CFP /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 5400 .. 6113 / #00ffff 2: 6114 .. 6116 / #00ffff / 1a 3: 6117 .. 6119 / #00ffff" /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" promoter complement(join(6130..6215,6243..6248,6249..6254)) /label=hsp70 mini /note="Drosophila melanogaster hsp70Bb promoter" /note="This reverse directional feature has 3 segments: 1: 6130 .. 6215 / #ffffff / align to 3xP3 2: 6243 .. 6248 / #cc99ff / TATA box 3: 6249 .. 6254 / #ffffff" misc_feature 6261..6378 /label=3xTpnC41C_PCR /note="color: #a6acb3" misc_feature complement(join(6274..6283,6286..6301,6304..6313,6316..6331, 6334..6343,6346..6361)) /label=3xTpnC41C /note="Expression of the Troponin C at 41C Gene in Adult Drosophila Tubular Muscles Depends upon Both Positive and Negative Regulatory Inputs Maria B. Chechenova, Sara Maes, Richard M. Cripps Published: December 7, 2015 '>https://doi.org/10.1371/journal.pone.0144615" /note="This reverse directional feature has 6 segments: 1: 6274 .. 6283 / #ff00ff / MEF2 2: 6286 .. 6301 / #a6acb3 / SM3 3: 6304 .. 6313 / #ff00ff / MEF2 4: 6316 .. 6331 / #a6acb3 / SM3 5: 6334 .. 6343 / #ff00ff / MEF2 6: 6346 .. 6361 / #a6acb3 / SM3" misc_feature complement(join(9305..9506,1..86)) /label=PhiC31 attB /note="color: #a6acb3; direction: LEFT" misc_feature complement(join(9313..9506,1..86)) /label=attB /note="color: #a6acb3; direction: LEFT" ORIGIN 1 gatgggtgag gtggagtacg cgcccgggga gcccaagggc acgccctggc acccgcaccg 61 cggcttcgag accgtgacct acatcgaggc ccttggatcc ctaacttccg tgacccgttc 121 acaaatacca tttccctaaa aataaccttc acaaatacca tttccctaaa aataaccttc 181 acaaatacca tttccctaaa aataaagcgg agactctaag cttatcgatt tcgaaccctc 241 gaccgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa ttcaaacaag 301 caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc tgaacaagct 361 aaacaatcgg ctcgaagccg gtcgccacca tggcctcctc cgaggacgtc atcaaggagt 421 tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg 481 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg 541 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg 601 tgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct 661 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact 721 cctccctcca ggacggctcc ttcatctaca aggtgaagtt catcggcgtg aacttcccct 781 ccgacggccc cgtaatgcag aagaagacta tgggctggga ggcgtccacc gagcgcctgt 841 acccccgcga cggcgtgctg aagggcgaga tccacaaggc cctgaagctg aaggacggcg 901 gccactacct ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg 961 gctactacta cgtggactcc aagctggaca tcacctccca caacgaggac tacaccatcg 1021 tggagcagta cgagcgcgcc gagggccgcc accacctgtt cctgtagggg ccgcgactct 1081 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 1141 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 1201 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 1261 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttac ctgcaggagt 1321 aaactcgtcg acggtatcga taagcgctag cttttttgct cacctgtgat tgctcctact 1381 caaatacaaa aacatcaaat tttctgtcaa taaagcatat ttatttatat ttattttaca 1441 ggaaagaatt ccttttaaag tgtattttaa cctataatga aaaacgatta aaaaaaatac 1501 ataaaataat tcgaaaattt ttgaatagcc caggttgata aaaattcatt tcatacgttt 1561 tataacttat gcccctaagt attttttgac catagtgttt caattctaca ttaattttac 1621 agagtagaat gaaacgccac ctactcagcc aagaggcgaa aaggttagct cgccaagcag 1681 agagggcgcc agtgctcact actttttata attctcaact tctttttcca gactcagttc 1741 gtatatatag acctattttc aatttaacgt cggccgcagt ccgatcatcg gatgtcttag 1801 agctatgctg gaaacagcat agcaagttaa gataaggcta gtccgttatc aacttgaaaa 1861 agtggcaccg agtcggtgct tttttatcga attcctgcag cccggtacct cgacggtaac 1921 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 1981 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 2041 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 2101 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 2161 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 2221 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 2281 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 2341 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 2401 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 2461 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 2521 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 2581 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 2641 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 2701 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 2761 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 2821 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 2881 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 2941 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 3001 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 3061 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 3121 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 3181 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 3241 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 3301 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 3361 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 3421 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 3481 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 3541 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 3601 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 3661 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaag 3721 cggccgctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg 3781 gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga 3841 ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt 3901 ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggaggcgcgc 3961 cctagttcca gtgaaatcca agcattttct aaattaaatg tattcttatt attatagttg 4021 ttatttttga tatatataaa caacactatt atgcccacca tttttttgag atgcatctac 4081 acaaggaaca aacactggat gtcactttca gttcaaattg taacgctaat cactccgaac 4141 aggtcacaaa aaattacctt aaaaagtcat aatattaaat tagaataaat atagctgtga 4201 gggaaatata tacaaatata ttggagcaaa taaattgtac atacaaatat ttattactaa 4261 tttctattga gacgaaatga accactcgga accatttgag cgaaccgaat cgcgcggaac 4321 taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg 4381 gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg 4441 cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta gcgataaacg 4501 agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca aatccaaagt 4561 ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata tcttgttttt 4621 attggcacaa atataattac attagctttt tgagggggca ataaacagta aacacgatgg 4681 taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc tactccttgc 4741 gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg atgagaatgg 4801 ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc tccaggatga 4861 ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg cccggctcca 4921 cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat gagaggtacg 4981 acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaagccg ccaaagagca 5041 ggaatggtat gataaccggc ggacccacag acagcgccat cgaggtcgag gagctggcgc 5101 aggatattag atatccgaag gacgttgaca cattggccac cagagtgacc agcgccaggc 5161 agttgaagaa gtgcagcact ccggcccgca gtccgatcat cggactagta aaagcaaaaa 5221 gcatttacct tgattgagat gttaattgtg ttggcaatta tcagtgcatg ctaagataca 5281 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa 5341 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaccggtt 5401 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg 5461 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag 5521 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg 5581 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagtgggc cttgatgccg 5641 ttcttctgct tgtcggcggt gatatagacg ttgtggctga tgtagttgta ctccagcttg 5701 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc 5761 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag 5821 aagatggtac gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc 5881 ttcatgtggt cggggtagcg gctgaagcac tgcacgcccc aggtcagggt ggtcacgagg 5941 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg 6001 taggtggcat cgccctcgcc ctcgccggac acgctgaacc tgtggccgtt tacgtcgccg 6061 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg 6121 ttgacgcgtc cgattgttta gcttgttcag ctgcgcttgt ttatttgctt agctttcgct 6181 tagcgacgtg ttcactttgc ttgtttgaat tgaattgtcg ctccgtagac gaagcgcctc 6241 tatttatact ccggcgtacg tagagtctcc gctttatttt tagggaaatg gtatttgtga 6301 aggttatttt tagggaaatg gtatttgtga aggttatttt tagggaaatg gtatttgtga 6361 acgggtcacg gaagttagcc taggtctcga gaagggcctg tcgggcccga taggcaatcg 6421 ccgtgaagac cagtggcact gtgagaaaaa gcggcaattc ggcaatcgtt ttgcccagaa 6481 agtatgtgtc acagcgataa agtcgacttc gggcctccct cataaaaact ggcagctctg 6541 aggtgaacac ctaaatcgaa tcgattcatt agaaagttag taaattattg aaatgcaaat 6601 gtattctaaa catgacttac atttatcgtg gcaaagacgt tttgaaaggt catgttggtc 6661 aggaagagga agatggctcc gttgatattc atcacaccca cttgcgtgag ttgttggccc 6721 aaaaagatga ggccaatcaa gatggcaacc atctgcaaat taaaatgtta ctcgcatctc 6781 attaatattc gcgagttaaa tgaaatttat ttatcttctg caaaactata aactatacat 6841 ctcattgaaa aaaactaaga agggtgtgga atcaggcaat tctatctaaa atctagcgaa 6901 tttgtttcca agaattgtaa gcgttatatc atttgtttcc actggaacca ctcaccgttg 6961 tctgaataag tcgcactttt acgaggagtg gttccttgag caccgacagc caggatcgcc 7021 acaggaccgc ccggaactgc atgaaccagg tggccttgta ggtgtaccca ttctccggct 7081 gctccagtgg cttctccaga tttttggtgg ccaacaactg ctccatatcc cgggctactt 7141 tgctaatggc aaaattgtcg catatcttgg cgatccgatc acgggactcg atctcccgtc 7201 cgggcacaac ggccaacacc tgtacgtaaa agtccgccgg attgtagttg gtaggacact 7261 gggcacccac gctggatagg agttgagatg ttatgtaata ctagataccc ttaataaaca 7321 catcgaactc actaggaaaa gaagtcgacg gcttcgctgg gagtgcccaa gaaagctacc 7381 ctgccctcgg ccatcagaag gatcttgtca aagagctcaa acagctcgga agacggctga 7441 tgaatggtca ggatgacggt cttgcccttc tgcgacagct tcttcagcac ctggacgacg 7501 ctgtgggcgg taaaggagtc cagtccggag gtgggctcat cgcagatcag aagcggcgga 7561 tcggttagag cctcggaggc gaatgccaga cgcttccttt ctccgccgga cagacctttc 7621 accctgccgg gcacaccgat gatcgtgtgc tgacatttgc tgagcgaaag ctcctggatc 7681 acctgatcca cgcgggccac tcgctgccga taggtcagat gtcgtggcat ccgcaccatg 7741 gcttggaaaa tcaggtgttc cctggccgtt agggagccga taaagaggtc atcctgctgg 7801 acataggcgc acctggcctg catctccttg gcgtccacag gttggccatt gagcagtcgc 7861 atcccggatg gcgatacttg gatgccctgc ggcgatcgaa aggcaagggc attcagcagg 7921 gtcgtctttc cggcaccgga actgcccatc acggccaaaa gttcgcccgg ataggccacg 7981 ccgcaaactg agtttcaaat tggtaattgg accctttatt aagatttcac acagatcagc 8041 cgactgcgaa tagaaactca ccgttcttga gcaaatgttt cctgggcgcc ggtatgtgtc 8101 gctcgttgca gaatagtccg cgtgtccggt tgaccagctg ccgccatccg gagcccggct 8161 gattgaccgc cccaaagatg tccatattgt gccaggcata ggtgaggttc tcggctagtt 8221 ggccgctccc tgaaccggag tcctccggcg gactgggtgg ccggagcgtg ccgtagtttt 8281 tggcctgccc gaagccctgg ttaatgcagc tctgcgaagc cgctccgctg tcaccctgca 8341 atgatagggg atctcaaata tcaactacta gcgttatgct catctaaccc cgaacaaaaa 8401 gtaccccgaa gtatcctacg aagtaggttt atacttttat ttattttttg tgcatctagg 8461 atcagcttaa aatatctggt tgttatattt tttgtaaaaa agaatatagt cgaaaatgaa 8521 tgcctttaga tgtcttgatc atgatatgat ctcaaaaatt gtcttatata gcgagaacag 8581 ctaccagaat aatctgtttc gtgtcactat ttgtttgtgc aattgcggtt tgggattttt 8641 gtgggtcgca gttctcacgc cgcatacaat ttgatgttgc aatcgcagtt cctatagatc 8701 aagtgaactt aagatgtatg cacatgtact actcacattg ttcagatgct cggcagatgg 8761 gtgtttgctg cctccgcgaa ttaatagctc ctgatcctct tggcccattg ccgggatttt 8821 tcacactttc ccctgcttac ccacccaaaa ccaatcacca ccccaatcac tcaaaaaaca 8881 aacaaaaata agaagcgaga ggagttttgg cacagcactt tgtgtttaat tgatggcgta 8941 aaccgcttgg agcttcgtca cgaaaccgct gacaaaatgc aactgaaggc ggacattgac 9001 gctacgtaac gctacaaacg gtggcgaaag agatagcgga cgcagcggcg aaagagacgg 9061 cgatatttct gtggacagag aaggaggcaa acagcgctga ctttgagtgg aatgtcattt 9121 tgagtgagag gtaatcgaaa gaacctggta catcaaatac ccttggatcg aagtaaattt 9181 aaaactgatc agataagttc aatgatatcc agtgcagtaa aaaaaaaaaa tgtttttttt 9241 atctactttc cgcaaaaatg ggttttatta acttacatac atactaggcg cgccgaagca 9301 tatgttcggc ttgtcgacat gcccgccgtg accgtcgaga acccgctgac gctgccccgc 9361 gtatccgcac ccgccgacgc cgtcgcacgt cccgtgctca ccgtgaccac cgcgcccagc 9421 ggtttcgagg gcgagggctt cccggtgcgc cgcgcgttcg ccgggatcaa ctaccgccac 9481 ctcgacccgt tcatcatgat ggacca //