LOCUS SuRe.AD2(Bxb1)1 9640 bp DNA circular SYN 26-APR-2025 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9640) AUTHORS Junjie Luo TITLE Direct Submission JOURNAL Exported Apr 26, 2025 from SnapGene 8.0.3 https://www.snapgene.com FEATURES Location/Qualifiers source 1..9640 /mol_type="other DNA" /note="color: #ffffff" /organism="synthetic DNA construct" protein_bind complement(1..70) /label=attB /bound_moiety="phage phi-C31 integrase" /note="attB site for the phi-C31 integrase (Groth et al., 2000)" /note="color: #31849b; direction: LEFT" misc_feature complement(join(72..74,75..77,78..94)) /label=gRNAu target /label=attB_R1_gRNA /note="This reverse directional feature has 3 segments: 1: 72 .. 74 / #00ff00 2: 75 .. 77 / #00ff00 3: 78 .. 94 / #00ff00" misc_feature 101..288 /label=2xr4_PCR /note="color: #a6acb3" promoter join(118..133,138..153,158..173,178..193) /label=r4 promoter /note="This forward directional feature has 4 segments: 1: 118 .. 133 / #ffffff 2: 138 .. 153 / #ffffff 3: 158 .. 173 / #ffffff 4: 178 .. 193 / #ffffff" promoter join(200..215,220..235,240..255,260..275) /label=r4 promoter /note="This forward directional feature has 4 segments: 1: 200 .. 215 / #ffffff 2: 220 .. 235 / #ffffff 3: 240 .. 255 / #ffffff 4: 260 .. 275 / #ffffff" promoter join(316..321,322..327,355..440) /label=hsp70 mini /note="Drosophila melanogaster hsp70Bb promoter" /note="This forward directional feature has 3 segments: 1: 316 .. 321 / #ffffff 2: 322 .. 327 / #cc99ff / TATA box 3: 355 .. 440 / #ffffff / align to 3xP3" regulatory 454..463 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /note="color: #a6acb3" /regulatory_class="other" CDS 460..1137 /codon_start=1 /product="rapidly maturing tetrameric variant of DsRed fluorescent protein (Bevis and Glick, 2002)" /label=DsRed-Express /note="mammalian codon-optimized" /note="color: #ff0000" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH LFL" polyA_signal 1258..1379 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" promoter 1422..1842 /label=dU6-3 promoter /note="RNA polymerase III promoter for Drosophila U6-3 snRNA (Port et al., 2014)" /note="color: #ffffff; direction: RIGHT" misc_feature join(1844..1860,1861..1863) /label=gRNAd /label=w_chiRNA_f2(core) /note="gRNAd (targets mini-w+ and makes w null) - CCGCAGTCCGATCATCGGATNGG from white eraser. sequenced" /note="This forward directional feature has 2 segments: 1: 1844 .. 1860 / #00ff00 2: 1861 .. 1863 / #00ff00" misc_RNA 1864..1949 /label=LgRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" /note="color: #00ccff; direction: RIGHT" rep_origin complement(2050..2638) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(join(2809..3600,3601..3669)) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 2809 .. 3600 / #ccffcc 2: 3601 .. 3669 / #ccffcc / signal sequence Cleavage site after base 3600" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMISTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 3469 /label=C->A /note="color: #a6acb3" promoter complement(3670..3774) /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: LEFT" misc_feature complement(join(4032..5272,6543..9420)) /label=mini-w with AD2(Bxb1)1 insertion /note="This reverse directional feature has 2 segments: 1: 4032 .. 5272 / #ff0000 2: 6543 .. 9420 / #ff0000" misc_feature 4794..4813 /label=gRNAd(old version) /label=w_ApaI_gRNA /note="gRNAd ('old', targets mini-w+ and makes w hypomorph) - TGTCGGCTACTCCTTGCGTCNGG " /note="color: #00ff00; direction: RIGHT" misc_feature join(5256..5272,6543..6545,6546..6548) /label=gRNAd target with AD2(Bxb1)1 insertion /label=wChi2R2_LgRNA_0_off /note="This forward directional feature has 3 segments: 1: 5256 .. 5272 / #00ff00 2: 6543 .. 6545 / #00ff00 3: 6546 .. 6548 / #00ff00" misc_feature join(5273..5279,5280..5329,5330..5335,5336..6495, 6496..6542) /label=[AD2_B^{Bxb1}] /note="This forward directional feature has 5 segments: 1: 5273 .. 5279 / #ffffff 2: 5280 .. 5329 / #31849b / attB[Bxb1] 3: 5330 .. 5335 / #ffffff 4: 5336 .. 6495 / #00ffff / 2xr4-CFP 5: 6496 .. 6542 / #ffffff" misc_feature complement(5280..5329) /label=attB(Bxb1) /note="http://flybase.org/reports/FBto0000359 The attB(Bxb1) site (5'-TCGGCCGGCTTGTCGACGACGgcggtctcCGTCGTCAGGATCATCCGGGC-3') is one of the two target sites of the Bxb1:int integrase. Bxb1:int mediates recombination between two non-identical target sites, attP(Bxb1) and attB(Bxb1), each of which contains a short integration core flanked by imperfect inverted repeats. Exchange between the attP(Bxb1)/attB(Bxb1) pair results in the formation of hybrid sites that are no longer a substrate of the Bxb1:int integrase, and thus the exchange reaction driven by Bxb1:int is undirectional (reviewed in PMID:21195181, FBrf0231034). The recombination event catalyzed by Bxb1:int results in genetic modification, the nature of which is influenced by the relative orientation (direct or inverted), and location of the two target sites. The types of possible modification include deletion of DNA, generation of chromosomal rearrangements, integration of DNA into the genome, and replacement of genetic material with that from a donor plasmid using recombination-mediated cassette exchange (RMCE) (reviewed in FBrf0231034)." /note="color: #31849b; direction: LEFT" polyA_signal 5336..5457 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" CDS complement(join(5464..6177,6178..6180,6181..6183)) /codon_start=1 /product="cyan variant of GFP" /label=CFP /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 5464 .. 6177 / #00ffff 2: 6178 .. 6180 / #00ffff / 1a 3: 6181 .. 6183 / #00ffff" /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" promoter complement(join(6194..6279,6307..6312,6313..6318)) /label=hsp70 mini /note="Drosophila melanogaster hsp70Bb promoter" /note="This reverse directional feature has 3 segments: 1: 6194 .. 6279 / #ffffff / align to 3xP3 2: 6307 .. 6312 / #cc99ff / TATA box 3: 6313 .. 6318 / #ffffff" misc_feature 6325..6512 /label=2xr4_PCR /note="color: #a6acb3" promoter complement(join(6338..6353,6358..6373,6378..6393, 6398..6413)) /label=r4 promoter /note="This reverse directional feature has 4 segments: 1: 6338 .. 6353 / #ffffff 2: 6358 .. 6373 / #ffffff 3: 6378 .. 6393 / #ffffff 4: 6398 .. 6413 / #ffffff" promoter complement(join(6420..6435,6440..6455,6460..6475, 6480..6495)) /label=r4 promoter /note="This reverse directional feature has 4 segments: 1: 6420 .. 6435 / #ffffff 2: 6440 .. 6455 / #ffffff 3: 6460 .. 6475 / #ffffff 4: 6480 .. 6495 / #ffffff" misc_feature 8503 /label=T->A /note="color: #a6acb3" misc_feature 8799 /label=T->G /note="color: #a6acb3" misc_feature complement(join(9439..9640,1..86)) /label=PhiC31 attB /note="color: #a6acb3; direction: LEFT" misc_feature complement(join(9447..9640,1..86)) /label=attB /note="color: #a6acb3; direction: LEFT" ORIGIN 1 gatgggtgag gtggagtacg cgcccgggga gcccaagggc acgccctggc acccgcaccg 61 cggcttcgag accgtgacct acatcgaggc ccttggatcc ctaacttccg tgacccgtta 121 aaataatcag gcgtagatta aaataatcag gcggtcatta aaataatcag gcggagatta 181 aaataatcag gcgatgcatt taaaataatc aggcgtagat taaaataatc aggcggtcat 241 taaaataatc aggcggagat taaaataatc aggcgagcgg agactctaag cttatcgatt 301 tcgaaccctc gaccgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa 361 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc 421 tgaacaagct aaacaatcgg ctcgaagccg gtcgccacca tggcctcctc cgaggacgtc 481 atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc 541 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag 601 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac 661 ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc 721 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg 781 acccaggact cctccctcca ggacggctcc ttcatctaca aggtgaagtt catcggcgtg 841 aacttcccct ccgacggccc cgtaatgcag aagaagacta tgggctggga ggcgtccacc 901 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccacaaggc cctgaagctg 961 aaggacggcg gccactacct ggtggagttc aagtccatct acatggccaa gaagcccgtg 1021 cagctgcccg gctactacta cgtggactcc aagctggaca tcacctccca caacgaggac 1081 tacaccatcg tggagcagta cgagcgcgcc gagggccgcc accacctgtt cctgtagggg 1141 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 1201 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 1261 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 1321 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttac 1381 ctgcaggagt aaactcgtcg acggtatcga taagcgctag cttttttgct cacctgtgat 1441 tgctcctact caaatacaaa aacatcaaat tttctgtcaa taaagcatat ttatttatat 1501 ttattttaca ggaaagaatt ccttttaaag tgtattttaa cctataatga aaaacgatta 1561 aaaaaaatac ataaaataat tcgaaaattt ttgaatagcc caggttgata aaaattcatt 1621 tcatacgttt tataacttat gcccctaagt attttttgac catagtgttt caattctaca 1681 ttaattttac agagtagaat gaaacgccac ctactcagcc aagaggcgaa aaggttagct 1741 cgccaagcag agagggcgcc agtgctcact actttttata attctcaact tctttttcca 1801 gactcagttc gtatatatag acctattttc aatttaacgt cggccgcagt ccgatcatcg 1861 gatgtcttag agctatgctg gaaacagcat agcaagttaa gataaggcta gtccgttatc 1921 aacttgaaaa agtggcaccg agtcggtgct tttttatcga attcctgcag cccggtacct 1981 cgacggtaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 2041 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 2101 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 2161 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 2221 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 2281 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 2341 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 2401 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 2461 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 2521 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 2581 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 2641 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 2701 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 2761 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 2821 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 2881 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 2941 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 3001 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 3061 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 3121 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 3181 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 3241 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 3301 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 3361 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 3421 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctaa tcattggaaa 3481 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 3541 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 3601 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 3661 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 3721 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 3781 tccccgaaag cggccgctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc 3841 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa 3901 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg 3961 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc 4021 ggaggcgcgc cctagttcca gtgaaatcca agcattttct aaattaaatg tattcttatt 4081 attatagttg ttatttttga tatatataaa caacactatt atgcccacca tttttttgag 4141 atgcatctac acaaggaaca aacactggat gtcactttca gttcaaattg taacgctaat 4201 cactccgaac aggtcacaaa aaattacctt aaaaagtcat aatattaaat tagaataaat 4261 atagctgtga gggaaatata tacaaatata ttggagcaaa taaattgtac atacaaatat 4321 ttattactaa tttctattga gacgaaatga accactcgga accatttgag cgaaccgaat 4381 cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga 4441 gagcgggtgg gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat 4501 gaagagggcg cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta 4561 gcgataaacg agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca 4621 aatccaaagt ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata 4681 tcttgttttt attggcacaa atataattac attagctttt tgagggggca ataaacagta 4741 aacacgatgg taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc 4801 tactccttgc gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg 4861 atgagaatgg ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc 4921 tccaggatga ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg 4981 cccggctcca cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat 5041 gagaggtacg acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaagccg 5101 ccaaagagca ggaatggtat gataaccggc ggacccacag acagcgccat cgaggtcgag 5161 gagctggcgc aggatattag atatccgaag gacgttgaca cattggccac cagagtgacc 5221 agcgccaggc agttgaagaa gtgcagcact ccggcccgca gtccgatcat cggactagtg 5281 cccggatgat cctgacgacg gagaccgccg tcgtcgacaa gccggccgag catgctaaga 5341 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 5401 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttacc 5461 ggtttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag 5521 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag 5581 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg 5641 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt gggccttgat 5701 gccgttcttc tgcttgtcgg cggtgatata gacgttgtgg ctgatgtagt tgtactccag 5761 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt 5821 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt 5881 gaagaagatg gtacgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg 5941 ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccccaggtca gggtggtcac 6001 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt 6061 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacctgtggc cgtttacgtc 6121 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac 6181 catgttgacg cgtccgattg tttagcttgt tcagctgcgc ttgtttattt gcttagcttt 6241 cgcttagcga cgtgttcact ttgcttgttt gaattgaatt gtcgctccgt agacgaagcg 6301 cctctattta tactccggcg tacgtagagt ctccgctcgc ctgattattt taatctccgc 6361 ctgattattt taatgaccgc ctgattattt taatctacgc ctgattattt taaatgcatc 6421 gcctgattat tttaatctcc gcctgattat tttaatgacc gcctgattat tttaatctac 6481 gcctgattat tttaacgggt cacggaagtt agcctaggtc tcgagaaggg cctgtcgggc 6541 ccgataggca atcgccgtga agaccagtgg cactgtgaga aaaagcggca attcggcaat 6601 cgttttgccc agaaagtatg tgtcacagcg ataaagtcga cttcgggcct ccctcataaa 6661 aactggcagc tctgaggtga acacctaaat cgaatcgatt cattagaaag ttagtaaatt 6721 attgaaatgc aaatgtattc taaacatgac ttacatttat cgtggcaaag acgttttgaa 6781 aggtcatgtt ggtcaggaag aggaagatgg ctccgttgat attcatcaca cccacttgcg 6841 tgagttgttg gcccaaaaag atgaggccaa tcaagatggc aaccatctgc aaattaaaat 6901 gttactcgca tctcattaat attcgcgagt taaatgaaat ttatttatct tctgcaaaac 6961 tataaactat acatctcatt gaaaaaaact aagaagggtg tggaatcagg caattctatc 7021 taaaatctag cgaatttgtt tccaagaatt gtaagcgtta tatcatttgt ttccactgga 7081 accactcacc gttgtctgaa taagtcgcac ttttacgagg agtggttcct tgagcaccga 7141 cagccaggat cgccacagga ccgcccggaa ctgcatgaac caggtggcct tgtaggtgta 7201 cccattctcc ggctgctcca gtggcttctc cagatttttg gtggccaaca actgctccat 7261 atcccgggct actttgctaa tggcaaaatt gtcgcatatc ttggcgatcc gatcacggga 7321 ctcgatctcc cgtccgggca caacggccaa cacctgtacg taaaagtccg ccggattgta 7381 gttggtagga cactgggcac ccacgctgga taggagttga gatgttatgt aatactagat 7441 acccttaata aacacatcga actcactagg aaaagaagtc gacggcttcg ctgggagtgc 7501 ccaagaaagc taccctgccc tcggccatca gaaggatctt gtcaaagagc tcaaacagct 7561 cggaagacgg ctgatgaatg gtcaggatga cggtcttgcc cttctgcgac agcttcttca 7621 gcacctggac gacgctgtgg gcggtaaagg agtccagtcc ggaggtgggc tcatcgcaga 7681 tcagaagcgg cggatcggtt agagcctcgg aggcgaatgc cagacgcttc ctttctccgc 7741 cggacagacc tttcaccctg ccgggcacac cgatgatcgt gtgctgacat ttgctgagcg 7801 aaagctcctg gatcacctga tccacgcggg ccactcgctg ccgataggtc agatgtcgtg 7861 gcatccgcac catggcttgg aaaatcaggt gttccctggc cgttagggag ccgataaaga 7921 ggtcatcctg ctggacatag gcgcacctgg cctgcatctc cttggcgtcc acaggttggc 7981 cattgagcag tcgcatcccg gatggcgata cttggatgcc ctgcggcgat cgaaaggcaa 8041 gggcattcag cagggtcgtc tttccggcac cggaactgcc catcacggcc aaaagttcgc 8101 ccggataggc cacgccgcaa actgagtttc aaattggtaa ttggaccctt tattaagatt 8161 tcacacagat cagccgactg cgaatagaaa ctcaccgttc ttgagcaaat gtttcctggg 8221 cgccggtatg tgtcgctcgt tgcagaatag tccgcgtgtc cggttgacca gctgccgcca 8281 tccggagccc ggctgattga ccgccccaaa gatgtccata ttgtgccagg cataggtgag 8341 gttctcggct agttggccgc tccctgaacc ggagtcctcc ggcggactgg gtggccggag 8401 cgtgccgtag tttttggcct gcccgaagcc ctggttaatg cagctctgcg aagccgctcc 8461 gctgtcaccc tgcaatgata ggggatctca aatatcaact acaagcgtta tgctcatcta 8521 accccgaaca aaaagtaccc cgaagtatcc tacgaagtag gtttatactt ttatttattt 8581 tttgtgcatc taggatcagc ttaaaatatc tggttgttat attttttgta aaaaagaata 8641 tagtcgaaaa tgaatgcctt tagatgtctt gatcatgata tgatctcaaa aattgtctta 8701 tatagcgaga acagctacca gaataatctg tttcgtgtca ctatttgttt gtgcaattgc 8761 ggtttgggat ttttgtgggt cgcagttctc acgccgcaga caatttgatg ttgcaatcgc 8821 agttcctata gatcaagtga acttaagatg tatgcacatg tactactcac attgttcaga 8881 tgctcggcag atgggtgttt gctgcctccg cgaattaata gctcctgatc ctcttggccc 8941 attgccggga tttttcacac tttcccctgc ttacccaccc aaaaccaatc accaccccaa 9001 tcactcaaaa aacaaacaaa aataagaagc gagaggagtt ttggcacagc actttgtgtt 9061 taattgatgg cgtaaaccgc ttggagcttc gtcacgaaac cgctgacaaa atgcaactga 9121 aggcggacat tgacgctacg taacgctaca aacggtggcg aaagagatag cggacgcagc 9181 ggcgaaagag acggcgatat ttctgtggac agagaaggag gcaaacagcg ctgactttga 9241 gtggaatgtc attttgagtg agaggtaatc gaaagaacct ggtacatcaa atacccttgg 9301 atcgaagtaa atttaaaact gatcagataa gttcaatgat atccagtgca gtaaaaaaaa 9361 aaaatgtttt ttttatctac tttccgcaaa aatgggtttt attaacttac atacatacta 9421 ggcgcgccga agcatatgtt cggcttgtcg acatgcccgc cgtgaccgtc gagaacccgc 9481 tgacgctgcc ccgcgtatcc gcacccgccg acgccgtcgc acgtcccgtg ctcaccgtga 9541 ccaccgcgcc cagcggtttc gagggcgagg gcttcccggt gcgccgcgcg ttcgccggga 9601 tcaactaccg ccacctcgac ccgttcatca tgatggacca //