LOCUS SuRe.AD2(PhiC31) 10068 bp DNA circular SYN 26-APR-2025 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10068) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 26, 2025 from SnapGene 8.0.3 https://www.snapgene.com FEATURES Location/Qualifiers source 1..10068 /mol_type="other DNA" /note="color: #ffffff" /organism="synthetic DNA construct" promoter 76..490 /label=U6:3 promoter /note="color: #ffffff; direction: RIGHT" gene complement(join(745..1523,2828..6167)) /label=mini w+ (split) /label=mini w+ (Janelia) /note="This reverse directional feature has 2 segments: 1: 745 .. 1523 / #ff0000 2: 2828 .. 6167 / #ff0000" misc_feature join(1507..1523,2828..2830,2831..2833) /label=gRNAd(old) target with AD2(PhiC31)1 insertion /label=w_ApaI_gRNA(split) /note="This forward directional feature has 3 segments: 1: 1507 .. 1523 / #00ff00 2: 2828 .. 2830 / #00ff00 3: 2831 .. 2833 / #00ff00" misc_feature join(1524..1527,1528..1597,1598..2804,2805..2827) /label=[AD2,attB] /note="This forward directional feature has 4 segments: 1: 1524 .. 1527 / #ffffff 2: 1528 .. 1597 / #008080 / attB 3: 1598 .. 2804 / #00ff00 / 3xP3-GFP 4: 2805 .. 2827 / #ffffff" protein_bind complement(1528..1597) /label=attB /bound_moiety="phage phi-C31 integrase" /note="attB site for the phi-C31 integrase (Groth et al., 2000)" /note="color: #31849b; direction: LEFT" polyA_signal 1598..1719 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" CDS complement(join(1860..2573,2574..2576,2577..2579)) /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 1860 .. 2573 / #05fd14 2: 2574 .. 2576 / #05fd14 / 1a 3: 2577 .. 2579 / #05fd14" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" regulatory 2576..2585 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /note="color: #a6acb3" /regulatory_class="other" promoter complement(join(2599..2684,2712..2717,2718..2725,2758..2768, 2773..2783,2789..2799,2800..2804)) /label=3xP3 /note="Direct regulation of rhodopsin 1 byPax-6/eyeless in Drosophila: evidencefor a conserved functionin photoreceptors: TAATYNRATTA" /note="This reverse directional feature has 7 segments: 1: 2599 .. 2684 / #ffffff / transcription starting 2: 2712 .. 2717 / #00ff00 / TATA box 3: 2718 .. 2725 / #ffffff / HSP70 core 4: 2758 .. 2768 / #cc99ff / Pax6(ey) binding site 5: 2773 .. 2783 / #cc99ff / Pax6(ey) binding site 6: 2789 .. 2799 / #cc99ff / Pax6(ey) binding site 7: 2800 .. 2804 / #ffffff" CDS complement(join(2831..3431,3502..3633,3837..4152,4214..4868, 4943..5216,5618..5689)) /codon_start=1 /label=mini w+ CDS (Janelia)-ApaI /note="This feature has 6 segments: 1: 2831 .. 3431 / #993366 2: 3502 .. 3633 / #993366 3: 3837 .. 4152 / #993366 4: 4214 .. 4868 / #993366 5: 4943 .. 5216 / #993366 6: 5618 .. 5689 / #993366" /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRA" misc_feature join(3273..3289,3290..3292) /label=gRNAd /label=w_chiRNA_f2(core) /note="gRNAd (targets mini-w+ and makes w null) - CCGCAGTCCGATCATCGGATNGG from white eraser. sequenced" /note="This forward directional feature has 2 segments: 1: 3273 .. 3289 / #00ff00 2: 3290 .. 3292 / #00ff00" misc_feature complement(6182..6469) /label=PhiC31 attB /note="color: #a6acb3; direction: LEFT" misc_feature complement(6190..6469) /label=attB /note="color: #a6acb3; direction: LEFT" protein_bind complement(6384..6453) /label=attB /bound_moiety="phage phi-C31 integrase" /note="attB site for the phi-C31 integrase (Groth et al., 2000)" /note="color: #31849b; direction: LEFT" misc_feature complement(join(6455..6457,6458..6460,6461..6477)) /label=gRNAu target /label=attB_R1_gRNA /note="This reverse directional feature has 3 segments: 1: 6455 .. 6457 / #00ff00 2: 6458 .. 6460 / #00ff00 3: 6461 .. 6477 / #00ff00" promoter join(6493..6497,6498..6508,6514..6524,6529..6539,6572..6579, 6580..6585,6613..6698) /label=3xP3 /note="Direct regulation of rhodopsin 1 byPax-6/eyeless in Drosophila: evidencefor a conserved functionin photoreceptors: TAATYNRATTA" /note="This forward directional feature has 7 segments: 1: 6493 .. 6497 / #ffffff 2: 6498 .. 6508 / #cc99ff / Pax6(ey) binding site 3: 6514 .. 6524 / #cc99ff / Pax6(ey) binding site 4: 6529 .. 6539 / #cc99ff / Pax6(ey) binding site 5: 6572 .. 6579 / #ffffff / HSP70 core 6: 6580 .. 6585 / #00ff00 / TATA box 7: 6613 .. 6698 / #ffffff / transcription starting" regulatory 6712..6721 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /note="color: #a6acb3" /regulatory_class="other" CDS 6718..7395 /codon_start=1 /product="rapidly maturing tetrameric variant of DsRed fluorescent protein (Bevis and Glick, 2002)" /label=DsRed-Express /note="mammalian codon-optimized" /note="color: #ff0000" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH LFL" polyA_signal 7516..7637 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" promoter 7680..8100 /label=dU6-3 promoter /note="RNA polymerase III promoter for Drosophila U6-3 snRNA (Port et al., 2014)" /note="color: #ffffff; direction: RIGHT" misc_feature 8102..8121 /label=gRNAd(old version) /label=w_ApaI_gRNA /note="gRNAd ('old', targets mini-w+ and makes w hypomorph) - TGTCGGCTACTCCTTGCGTCNGG " /note="color: #00ff00; direction: RIGHT" misc_RNA 8122..8207 /label=LgRNA scaffold /note="long gRNA, removed tttt, extended loop guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" /note="color: #00ccff; direction: RIGHT" rep_origin complement(8313..8901) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(join(9072..9863,9864..9932)) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 9072 .. 9863 / #ccffcc 2: 9864 .. 9932 / #ccffcc / signal sequence Cleavage site after base 9863" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(9933..10037) /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: LEFT" ORIGIN 1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 61 cgtcttcaag aattcgctca cctgtgattg ctcctactca aatacaaaaa catcaaattt 121 tctgtcaata aagcatattt atttatattt attttacagg aaagaattcc ttttaaagtg 181 tattttaacc tataatgaaa aacgattaaa aaaaatacat aaaataattc gaaaattttt 241 gaatagccca ggttgataaa aattcatttc atacgtttta taacttatgc ccctaagtat 301 tttttgacca tagtgtttca attctacatt aattttacag agtagaatga aacgccacct 361 actcagccaa gaggcgaaaa ggttagctcg ccaagcagag agggcgccag tgctcactac 421 tttttataat tctcaacttc tttttccaga ctcagttcgt atatatagac ctattttcaa 481 tttaacgtcg ggtacctttt ttgcggccgc tggccacggg tgcgcatgat cgtgctcctg 541 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg 601 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca 661 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc 721 accattatgt tccggaggcg cgccctagtt ccagtgaaat ccaagcattt tctaaattaa 781 atgtattctt attattatag ttgttatttt tgatatatat aaacaacact attatgccca 841 ccattttttt gagatgcatc tacacaagga acaaacactg gatgtcactt tcagttcaaa 901 ttgtaacgct aatcactccg aacaggtcac aaaaaattac cttaaaaagt cataatatta 961 aattagaata aatatagctg tgagggaaat atatacaaat atattggagc aaataaattg 1021 tacatacaaa tatttattac taatttctat tgagacgaaa tgaaccactc ggaaccattt 1081 gagcgaaccg aatcgcgcgg aactaacgac agtcgctcca aggtcgtcga acaaaaggtg 1141 aatgtgttgc ggagagcggg tgggagacag cgaaagagca actacgaaac gtggtgtggt 1201 ggaggtgaat tatgaagagg gcgcgcgatt tgaaaagtat gtatataaaa aatatatccc 1261 ggtgttttat gtagcgataa acgagttttt gatgtaaggt atgcaggtgt gtaagtcttt 1321 tggttagaag acaaatccaa agtctacttg tggggatgtt cgaaggggaa atacttgtat 1381 tctataggtc atatcttgtt tttattggca caaatataat tacattagct ttttgagggg 1441 gcaataaaca gtaaacacga tggtaataat ggtaaaaaaa aaaaacaagc agttatttcg 1501 gatatatgtc ggctactcct tgcgtcggat gggtgaggtg gagtacgcgc ccggggagcc 1561 caagggcacg ccctggcacc cgcaccgcgg cttcgagtaa gatacattga tgagtttgga 1621 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt 1681 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat 1741 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac 1801 aaatgtggta tggctgatta tgatctagag tcgcggcctg agcgatcgct taggccgctt 1861 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg 1921 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag 1981 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg 2041 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg 2101 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg 2161 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc 2221 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag 2281 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc 2341 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg 2401 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg 2461 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg 2521 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg 2581 gtggcgaccg gcttcgagcc gattgtttag cttgttcagc tgcgcttgtt tatttgctta 2641 gctttcgctt agcgacgtgt tcactttgct tgtttgaatt gaattgtcgc tccgtagacg 2701 aagcgcctct atttatactc cggcggtcga gggttcgaaa tcgataagct tggatcctaa 2761 ttgaattagc tctaattgaa ttagtctcta attgaattag atccaattct tgaagacgaa 2821 agggcctgtc gggcccgaag tcttagagcc agatatgcga gcacccggaa gctcacgatg 2881 agaatggcca gacccacgta gtccagcggc agatcggcgg cggagaagtt aagcgtctcc 2941 aggatgacct tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct aatttcgccc 3001 ggctccacgt ccgcccattg gttaatcagc agaccctcgt tggcgtaacg gaaccatgag 3061 aggtacgaca accatttgag gtatactggc accgagcccg agttcaagaa gaagccgcca 3121 aagagcagga atggtatgat aaccggcgga cccacagaca gcgccatcga ggtcgaggag 3181 ctggcgcagg atattagata tccgaaggac gttgacacat tggccaccag agtgaccagc 3241 gccaggcagt tgaagaagtg cagcactccg gcccgcagtc cgatcatcgg ataggcaatc 3301 gccgtgaaga ccagtggcac tgtgagaaaa agcggcaatt cggcaatcgt tttgcccaga 3361 aagtatgtgt cacagcgata aagtcgactt cgggcctccc tcataaaaac tggcagctct 3421 gaggtgaaca cctaaatcga atcgattcat tagaaagtta gtaaattatt gaaatgcaaa 3481 tgtattctaa acatgactta catttatcgt ggcaaagacg ttttgaaagg tcatgttggt 3541 caggaagagg aagatggctc cgttgatatt catcacaccc acttgcgtga gttgttggcc 3601 caaaaagatg aggccaatca agatggcaac catctgcaaa ttaaaatgtt actcgcatct 3661 cattaatatt cgcgagttaa atgaaattta tttatcttct gcaaaactat aaactataca 3721 tctcattgaa aaaaactaag aagggtgtgg aatcaggcaa ttctatctaa aatctagcga 3781 atttgtttcc aagaattgta agcgttatat catttgtttc cactggaacc actcaccgtt 3841 gtctgaataa gtcgcacttt tacgaggagt ggttccttga gcaccgacag ccaggatcgc 3901 cacaggaccg cccggaactg catgaaccag gtggccttgt aggtgtaccc attctccggc 3961 tgctccagtg gcttctccag atttttggtg gccaacaact gctccatatc ccgggctact 4021 ttgctaatgg caaaattgtc gcatatcttg gcgatccgat cacgggactc gatctcccgt 4081 ccgggcacaa cggccaacac ctgtacgtaa aagtccgccg gattgtagtt ggtaggacac 4141 tgggcaccca cgctggatag gagttgagat gttatgtaat actagatacc cttaataaac 4201 acatcgaact cactaggaaa agaagtcgac ggcttcgctg ggagtgccca agaaagctac 4261 cctgccctcg gccatcagaa ggatcttgtc aaagagctca aacagctcgg aagacggctg 4321 atgaatggtc aggatgacgg tcttgccctt ctgcgacagc ttcttcagca cctggacgac 4381 gctgtgggcg gtaaaggagt ccagtccgga ggtgggctca tcgcagatca gaagcggcgg 4441 atcggttaga gcctcggagg cgaatgccag acgcttcctt tctccgccgg acagaccttt 4501 caccctgccg ggcacaccga tgatcgtgtg ctgacatttg ctgagcgaaa gctcctggat 4561 cacctgatcc acgcgggcca ctcgctgccg ataggtcaga tgtcgtggca tccgcaccat 4621 ggcttggaaa atcaggtgtt ccctggccgt tagggagccg ataaagaggt catcctgctg 4681 gacataggcg cacctggcct gcatctcctt ggcgtccaca ggttggccat tgagcagtcg 4741 catcccggat ggcgatactt ggatgccctg cggcgatcga aaggcaaggg cattcagcag 4801 ggtcgtcttt ccggcaccgg aactgcccat cacggccaaa agttcgcccg gataggccac 4861 gccgcaaact gagtttcaaa ttggtaattg gaccctttat taagatttca cacagatcag 4921 ccgactgcga atagaaactc accgttcttg agcaaatgtt tcctgggcgc cggtatgtgt 4981 cgctcgttgc agaatagtcc gcgtgtccgg ttgaccagct gccgccatcc ggagcccggc 5041 tgattgaccg ccccaaagat gtccatattg tgccaggcat aggtgaggtt ctcggctagt 5101 tggccgctcc ctgaaccgga gtcctccggc ggactgggtg gccggagcgt gccgtagttt 5161 ttggcctgcc cgaagccctg gttaatgcag ctctgcgaag ccgctccgct gtcaccctgc 5221 aatgataggg gatctcaaat atcaactact agcgttatgc tcatctaacc ccgaacaaaa 5281 agtaccccga agtatcctac gaagtaggtt tatactttta tttatttttt gtgcatctag 5341 gatcagctta aaatatctgg ttgttatatt ttttgtaaaa aagaatatag tcgaaaatga 5401 atgcctttag atgtcttgat catgatatga tctcaaaaat tgtcttatat agcgagaaca 5461 gctaccagaa taatctgttt cgtgtcacta tttgtttgtg caattgcggt ttgggatttt 5521 tgtgggtcgc agttctcacg ccgcatacaa tttgatgttg caatcgcagt tcctatagat 5581 caagtgaact taagatgtat gcacatgtac tactcacatt gttcagatgc tcggcagatg 5641 ggtgtttgct gcctccgcga attaatagct cctgatcctc ttggcccatt gccgggattt 5701 ttcacacttt cccctgctta cccacccaaa accaatcacc accccaatca ctcaaaaaac 5761 aaacaaaaat aagaagcgag aggagttttg gcacagcact ttgtgtttaa ttgatggcgt 5821 aaaccgcttg gagcttcgtc acgaaaccgc tgacaaaatg caactgaagg cggacattga 5881 cgctacgtaa cgctacaaac ggtggcgaaa gagatagcgg acgcagcggc gaaagagacg 5941 gcgatatttc tgtggacaga gaaggaggca aacagcgctg actttgagtg gaatgtcatt 6001 ttgagtgaga ggtaatcgaa agaacctggt acatcaaata cccttggatc gaagtaaatt 6061 taaaactgat cagataagtt caatgatatc cagtgcagta aaaaaaaaaa atgttttttt 6121 tatctacttt ccgcaaaaat gggttttatt aacttacata catactaggc gcgcccatat 6181 gttcggcttg tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct gccccgcgta 6241 tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc gcccagcggt 6301 ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta ccgccacctc 6361 gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg ggagcccaag 6421 ggcacgccct ggcacccgca ccgcggcttc gagaccgtga cctacatcga ggccctttcg 6481 tcttcaagaa ttggatctaa ttcaattaga gactaattca attagagcta attcaattag 6541 gatccaagct tatcgatttc gaaccctcga ccgccggagt ataaatagag gcgcttcgtc 6601 tacggagcga caattcaatt caaacaagca aagtgaacac gtcgctaagc gaaagctaag 6661 caaataaaca agcgcagctg aacaagctaa acaatcggct cgaagccggt cgccaccatg 6721 gcctcctccg aggacgtcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc 6781 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc 6841 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg 6901 tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac 6961 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 7021 ggcggcgtgg tgaccgtgac ccaggactcc tccctccagg acggctcctt catctacaag 7081 gtgaagttca tcggcgtgaa cttcccctcc gacggccccg taatgcagaa gaagactatg 7141 ggctgggagg cgtccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc 7201 cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac 7261 atggccaaga agcccgtgca gctgcccggc tactactacg tggactccaa gctggacatc 7321 acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcgccga gggccgccac 7381 cacctgttcc tgtaggggcc gcgactctag atcataatca gccataccac atttgtagag 7441 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 7501 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 7561 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 7621 ctcatcaatg tatcttacct gcaggagtaa actcgtcgac ggtatcgata agcgctagct 7681 tttttgctca cctgtgattg ctcctactca aatacaaaaa catcaaattt tctgtcaata 7741 aagcatattt atttatattt attttacagg aaagaattcc ttttaaagtg tattttaacc 7801 tataatgaaa aacgattaaa aaaaatacat aaaataattc gaaaattttt gaatagccca 7861 ggttgataaa aattcatttc atacgtttta taacttatgc ccctaagtat tttttgacca 7921 tagtgtttca attctacatt aattttacag agtagaatga aacgccacct actcagccaa 7981 gaggcgaaaa ggttagctcg ccaagcagag agggcgccag tgctcactac tttttataat 8041 tctcaacttc tttttccaga ctcagttcgt atatatagac ctattttcaa tttaacgtcg 8101 gtgtcggcta ctccttgcgt cgtcttagag ctatgctgga aacagcatag caagttaaga 8161 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttatcgaat 8221 tcctgcagcc cggcctacat cgtcgacggt aacatgtgag caaaaggcca gcaaaaggcc 8281 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 8341 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 8401 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 8461 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 8521 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 8581 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 8641 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 8701 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 8761 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 8821 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 8881 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 8941 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 9001 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 9061 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 9121 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 9181 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 9241 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 9301 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 9361 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 9421 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 9481 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 9541 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 9601 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 9661 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 9721 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 9781 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 9841 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 9901 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 9961 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 10021 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc //