LOCUS SuRe.AD2(Bxb1)2 9509 bp DNA circular SYN 26-APR-2025 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9509) AUTHORS Junjie Luo TITLE Direct Submission JOURNAL Exported Apr 26, 2025 from SnapGene 8.0.3 https://www.snapgene.com FEATURES Location/Qualifiers source 1..9509 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind complement(1..70) /label=attB /bound_moiety="phage phi-C31 integrase" /note="attB site for the phi-C31 integrase (Groth et al., 2000)" /note="color: #31849b; direction: LEFT" misc_feature complement(join(72..74,75..77,78..94)) /label=gRNAu target /label=attB_R1_gRNA /note="This reverse directional feature has 3 segments: 1: 72 .. 74 / #00ff00 2: 75 .. 77 / #00ff00 3: 78 .. 94 / #00ff00" misc_feature 101..288 /label=2xr4_PCR /note="color: #a6acb3" promoter join(118..133,138..153,158..173,178..193) /label=r4 promoter /note="Integrating sex- and tissue-specificregulauon within a single Drosophilaenhancer Wenqian An and Pieter C. Wensink 1 doi:10.1101/gad.9.2.256Genes & Dev. 1995. 9: 256-266 http://genesdev.cshlp.org.stanford.idm.oclc.org/content/9/2 /256.full.pdf+html" /note="This forward directional feature has 4 segments: 1: 118 .. 133 / #ffffff 2: 138 .. 153 / #ffffff 3: 158 .. 173 / #ffffff 4: 178 .. 193 / #ffffff" promoter join(200..215,220..235,240..255,260..275) /label=r4 promoter /note="Integrating sex- and tissue-specificregulauon within a single Drosophilaenhancer Wenqian An and Pieter C. Wensink 1 doi:10.1101/gad.9.2.256Genes & Dev. 1995. 9: 256-266 http://genesdev.cshlp.org.stanford.idm.oclc.org/content/9/2 /256.full.pdf+html" /note="This forward directional feature has 4 segments: 1: 200 .. 215 / #ffffff 2: 220 .. 235 / #ffffff 3: 240 .. 255 / #ffffff 4: 260 .. 275 / #ffffff" promoter join(316..321,322..327,355..440) /label=hsp70 mini /note="Drosophila melanogaster hsp70Bb promoter" /note="This forward directional feature has 3 segments: 1: 316 .. 321 / #ffffff 2: 322 .. 327 / #cc99ff / TATA box 3: 355 .. 440 / #ffffff / align to 3xP3" regulatory 454..463 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /note="color: #a6acb3" /regulatory_class="other" CDS 460..1137 /codon_start=1 /product="rapidly maturing tetrameric variant of DsRed fluorescent protein (Bevis and Glick, 2002)" /label=DsRed-Express /note="mammalian codon-optimized" /note="color: #ff0000" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH LFL" polyA_signal 1258..1379 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" promoter 1422..1842 /label=dU6-3 promoter /note="RNA polymerase III promoter for Drosophila U6-3 snRNA (Port et al., 2014)" /note="color: #ffffff; direction: RIGHT" misc_feature join(1844..1860,1861..1863) /label=gRNAd /label=w_chiRNA_f2(core) /note="gRNAd (targets mini-w+ and makes w null) - CCGCAGTCCGATCATCGGATNGG from white eraser. sequenced" /note="This forward directional feature has 2 segments: 1: 1844 .. 1860 / #00ff00 2: 1861 .. 1863 / #00ff00" misc_RNA 1864..1949 /label=LgRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" /note="color: #00ccff; direction: RIGHT" rep_origin complement(2050..2638) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(join(2809..3600,3601..3669)) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 2809 .. 3600 / #ccffcc 2: 3601 .. 3669 / #ccffcc / signal sequence Cleavage site after base 3600" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3670..3774) /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: LEFT" misc_feature complement(join(4032..5272,6412..9289)) /label=mini-w with AD2(Bxb1)2 insertion /note="This reverse directional feature has 2 segments: 1: 4032 .. 5272 / #ff0000 2: 6412 .. 9289 / #ff0000" misc_feature 4794..4813 /label=gRNAd(old version) /label=w_ApaI_gRNA /note="gRNAd ('old', targets mini-w+ and makes w hypomorph) - TGTCGGCTACTCCTTGCGTCNGG " /note="color: #00ff00; direction: RIGHT" misc_feature join(5256..5272,6412..6414,6415..6417) /label=gRNAd target with AD2(Bxb1)2 insertion /note="This forward directional feature has 3 segments: 1: 5256 .. 5272 / #00ff00 2: 6412 .. 6414 / #00ff00 3: 6415 .. 6417 / #00ff00" misc_feature join(5280..5329,5336..6381,6382..6411) /label=AD2(Bxb1)2 /note="This forward directional feature has 3 segments: 1: 5280 .. 5329 / #31849b / attB(Bxb1) 2: 5336 .. 6381 / #00ffff / 3xP3-CFP 3: 6382 .. 6411 / #ffffff" misc_feature complement(5280..5329) /label=attB(Bxb1) /note="http://flybase.org/reports/FBto0000359 The attB(Bxb1) site (5'-TCGGCCGGCTTGTCGACGACGgcggtctcCGTCGTCAGGATCATCCGGGC-3') is one of the two target sites of the Bxb1:int integrase. Bxb1:int mediates recombination between two non-identical target sites, attP(Bxb1) and attB(Bxb1), each of which contains a short integration core flanked by imperfect inverted repeats. Exchange between the attP(Bxb1)/attB(Bxb1) pair results in the formation of hybrid sites that are no longer a substrate of the Bxb1:int integrase, and thus the exchange reaction driven by Bxb1:int is undirectional (reviewed in PMID:21195181, FBrf0231034). The recombination event catalyzed by Bxb1:int results in genetic modification, the nature of which is influenced by the relative orientation (direct or inverted), and location of the two target sites. The types of possible modification include deletion of DNA, generation of chromosomal rearrangements, integration of DNA into the genome, and replacement of genetic material with that from a donor plasmid using recombination-mediated cassette exchange (RMCE) (reviewed in FBrf0231034)." /note="color: #31849b; direction: LEFT" polyA_signal 5336..5457 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" CDS complement(join(5464..6177,6178..6180,6181..6183)) /codon_start=1 /product="cyan variant of GFP" /label=CFP /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 5464 .. 6177 / #00ffff 2: 6178 .. 6180 / #00ffff / 1a 3: 6181 .. 6183 / #00ffff" /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" promoter complement(join(6194..6279,6307..6312,6313..6320,6335..6345, 6350..6360,6366..6376,6377..6381)) /label=3xP3(NgoMIV NaeI) /note="Direct regulation of rhodopsin 1 byPax-6/eyeless in Drosophila: evidencefor a conserved functionin photoreceptors: TAATYNRATTA" /note="This reverse directional feature has 7 segments: 1: 6194 .. 6279 / #ffffff / transcription starting 2: 6307 .. 6312 / #00ff00 / TATA box 3: 6313 .. 6320 / #ffffff / HSP70 core 4: 6335 .. 6345 / #cc99ff / Pax6(ey) binding site 5: 6350 .. 6360 / #cc99ff / Pax6(ey) binding site 6: 6366 .. 6376 / #cc99ff / Pax6(ey) binding site 7: 6377 .. 6381 / #ffffff" misc_feature complement(join(9308..9509,1..86)) /label=PhiC31 attB /note="color: #a6acb3; direction: LEFT" misc_feature complement(join(9316..9509,1..86)) /label=attB /note="color: #a6acb3; direction: LEFT" ORIGIN 1 gatgggtgag gtggagtacg cgcccgggga gcccaagggc acgccctggc acccgcaccg 61 cggcttcgag accgtgacct acatcgaggc ccttggatcc ctaacttccg tgacccgtta 121 aaataatcag gcgtagatta aaataatcag gcggtcatta aaataatcag gcggagatta 181 aaataatcag gcgatgcatt taaaataatc aggcgtagat taaaataatc aggcggtcat 241 taaaataatc aggcggagat taaaataatc aggcgagcgg agactctaag cttatcgatt 301 tcgaaccctc gaccgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa 361 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc 421 tgaacaagct aaacaatcgg ctcgaagccg gtcgccacca tggcctcctc cgaggacgtc 481 atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc 541 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag 601 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac 661 ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc 721 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg 781 acccaggact cctccctcca ggacggctcc ttcatctaca aggtgaagtt catcggcgtg 841 aacttcccct ccgacggccc cgtaatgcag aagaagacta tgggctggga ggcgtccacc 901 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccacaaggc cctgaagctg 961 aaggacggcg gccactacct ggtggagttc aagtccatct acatggccaa gaagcccgtg 1021 cagctgcccg gctactacta cgtggactcc aagctggaca tcacctccca caacgaggac 1081 tacaccatcg tggagcagta cgagcgcgcc gagggccgcc accacctgtt cctgtagggg 1141 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 1201 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 1261 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 1321 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttac 1381 ctgcaggagt aaactcgtcg acggtatcga taagcgctag cttttttgct cacctgtgat 1441 tgctcctact caaatacaaa aacatcaaat tttctgtcaa taaagcatat ttatttatat 1501 ttattttaca ggaaagaatt ccttttaaag tgtattttaa cctataatga aaaacgatta 1561 aaaaaaatac ataaaataat tcgaaaattt ttgaatagcc caggttgata aaaattcatt 1621 tcatacgttt tataacttat gcccctaagt attttttgac catagtgttt caattctaca 1681 ttaattttac agagtagaat gaaacgccac ctactcagcc aagaggcgaa aaggttagct 1741 cgccaagcag agagggcgcc agtgctcact actttttata attctcaact tctttttcca 1801 gactcagttc gtatatatag acctattttc aatttaacgt cggccgcagt ccgatcatcg 1861 gatgtcttag agctatgctg gaaacagcat agcaagttaa gataaggcta gtccgttatc 1921 aacttgaaaa agtggcaccg agtcggtgct tttttatcga attcctgcag cccggtacct 1981 cgacggtaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 2041 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 2101 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 2161 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 2221 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 2281 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 2341 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 2401 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 2461 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 2521 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 2581 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 2641 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 2701 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 2761 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 2821 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 2881 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 2941 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 3001 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 3061 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 3121 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 3181 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 3241 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 3301 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 3361 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 3421 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 3481 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 3541 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 3601 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 3661 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 3721 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 3781 tccccgaaag cggccgctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc 3841 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa 3901 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg 3961 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc 4021 ggaggcgcgc cctagttcca gtgaaatcca agcattttct aaattaaatg tattcttatt 4081 attatagttg ttatttttga tatatataaa caacactatt atgcccacca tttttttgag 4141 atgcatctac acaaggaaca aacactggat gtcactttca gttcaaattg taacgctaat 4201 cactccgaac aggtcacaaa aaattacctt aaaaagtcat aatattaaat tagaataaat 4261 atagctgtga gggaaatata tacaaatata ttggagcaaa taaattgtac atacaaatat 4321 ttattactaa tttctattga gacgaaatga accactcgga accatttgag cgaaccgaat 4381 cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga 4441 gagcgggtgg gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat 4501 gaagagggcg cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta 4561 gcgataaacg agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca 4621 aatccaaagt ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata 4681 tcttgttttt attggcacaa atataattac attagctttt tgagggggca ataaacagta 4741 aacacgatgg taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc 4801 tactccttgc gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg 4861 atgagaatgg ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc 4921 tccaggatga ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg 4981 cccggctcca cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat 5041 gagaggtacg acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaagccg 5101 ccaaagagca ggaatggtat gataaccggc ggacccacag acagcgccat cgaggtcgag 5161 gagctggcgc aggatattag atatccgaag gacgttgaca cattggccac cagagtgacc 5221 agcgccaggc agttgaagaa gtgcagcact ccggcccgca gtccgatcat cggactagtg 5281 cccggatgat cctgacgacg gagaccgccg tcgtcgacaa gccggccgag catgctaaga 5341 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 5401 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttacc 5461 ggtttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag 5521 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag 5581 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg 5641 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt gggccttgat 5701 gccgttcttc tgcttgtcgg cggtgatata gacgttgtgg ctgatgtagt tgtactccag 5761 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt 5821 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt 5881 gaagaagatg gtacgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg 5941 ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccccaggtca gggtggtcac 6001 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt 6061 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacctgtggc cgtttacgtc 6121 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac 6181 catgttgacg cgtccgattg tttagcttgt tcagctgcgc ttgtttattt gcttagcttt 6241 cgcttagcga cgtgttcact ttgcttgttt gaattgaatt gtcgctccgt agacgaagcg 6301 cctctattta tactccggcg gtctaaatgc cggctaattg aattagctct aattgaatta 6361 gtctctaatt gaattagatc ccctaggtct cgagaagggc ctgtcgggcc cgataggcaa 6421 tcgccgtgaa gaccagtggc actgtgagaa aaagcggcaa ttcggcaatc gttttgccca 6481 gaaagtatgt gtcacagcga taaagtcgac ttcgggcctc cctcataaaa actggcagct 6541 ctgaggtgaa cacctaaatc gaatcgattc attagaaagt tagtaaatta ttgaaatgca 6601 aatgtattct aaacatgact tacatttatc gtggcaaaga cgttttgaaa ggtcatgttg 6661 gtcaggaaga ggaagatggc tccgttgata ttcatcacac ccacttgcgt gagttgttgg 6721 cccaaaaaga tgaggccaat caagatggca accatctgca aattaaaatg ttactcgcat 6781 ctcattaata ttcgcgagtt aaatgaaatt tatttatctt ctgcaaaact ataaactata 6841 catctcattg aaaaaaacta agaagggtgt ggaatcaggc aattctatct aaaatctagc 6901 gaatttgttt ccaagaattg taagcgttat atcatttgtt tccactggaa ccactcaccg 6961 ttgtctgaat aagtcgcact tttacgagga gtggttcctt gagcaccgac agccaggatc 7021 gccacaggac cgcccggaac tgcatgaacc aggtggcctt gtaggtgtac ccattctccg 7081 gctgctccag tggcttctcc agatttttgg tggccaacaa ctgctccata tcccgggcta 7141 ctttgctaat ggcaaaattg tcgcatatct tggcgatccg atcacgggac tcgatctccc 7201 gtccgggcac aacggccaac acctgtacgt aaaagtccgc cggattgtag ttggtaggac 7261 actgggcacc cacgctggat aggagttgag atgttatgta atactagata cccttaataa 7321 acacatcgaa ctcactagga aaagaagtcg acggcttcgc tgggagtgcc caagaaagct 7381 accctgccct cggccatcag aaggatcttg tcaaagagct caaacagctc ggaagacggc 7441 tgatgaatgg tcaggatgac ggtcttgccc ttctgcgaca gcttcttcag cacctggacg 7501 acgctgtggg cggtaaagga gtccagtccg gaggtgggct catcgcagat cagaagcggc 7561 ggatcggtta gagcctcgga ggcgaatgcc agacgcttcc tttctccgcc ggacagacct 7621 ttcaccctgc cgggcacacc gatgatcgtg tgctgacatt tgctgagcga aagctcctgg 7681 atcacctgat ccacgcgggc cactcgctgc cgataggtca gatgtcgtgg catccgcacc 7741 atggcttgga aaatcaggtg ttccctggcc gttagggagc cgataaagag gtcatcctgc 7801 tggacatagg cgcacctggc ctgcatctcc ttggcgtcca caggttggcc attgagcagt 7861 cgcatcccgg atggcgatac ttggatgccc tgcggcgatc gaaaggcaag ggcattcagc 7921 agggtcgtct ttccggcacc ggaactgccc atcacggcca aaagttcgcc cggataggcc 7981 acgccgcaaa ctgagtttca aattggtaat tggacccttt attaagattt cacacagatc 8041 agccgactgc gaatagaaac tcaccgttct tgagcaaatg tttcctgggc gccggtatgt 8101 gtcgctcgtt gcagaatagt ccgcgtgtcc ggttgaccag ctgccgccat ccggagcccg 8161 gctgattgac cgccccaaag atgtccatat tgtgccaggc ataggtgagg ttctcggcta 8221 gttggccgct ccctgaaccg gagtcctccg gcggactggg tggccggagc gtgccgtagt 8281 ttttggcctg cccgaagccc tggttaatgc agctctgcga agccgctccg ctgtcaccct 8341 gcaatgatag gggatctcaa atatcaacta ctagcgttat gctcatctaa ccccgaacaa 8401 aaagtacccc gaagtatcct acgaagtagg tttatacttt tatttatttt ttgtgcatct 8461 aggatcagct taaaatatct ggttgttata ttttttgtaa aaaagaatat agtcgaaaat 8521 gaatgccttt agatgtcttg atcatgatat gatctcaaaa attgtcttat atagcgagaa 8581 cagctaccag aataatctgt ttcgtgtcac tatttgtttg tgcaattgcg gtttgggatt 8641 tttgtgggtc gcagttctca cgccgcatac aatttgatgt tgcaatcgca gttcctatag 8701 atcaagtgaa cttaagatgt atgcacatgt actactcaca ttgttcagat gctcggcaga 8761 tgggtgtttg ctgcctccgc gaattaatag ctcctgatcc tcttggccca ttgccgggat 8821 ttttcacact ttcccctgct tacccaccca aaaccaatca ccaccccaat cactcaaaaa 8881 acaaacaaaa ataagaagcg agaggagttt tggcacagca ctttgtgttt aattgatggc 8941 gtaaaccgct tggagcttcg tcacgaaacc gctgacaaaa tgcaactgaa ggcggacatt 9001 gacgctacgt aacgctacaa acggtggcga aagagatagc ggacgcagcg gcgaaagaga 9061 cggcgatatt tctgtggaca gagaaggagg caaacagcgc tgactttgag tggaatgtca 9121 ttttgagtga gaggtaatcg aaagaacctg gtacatcaaa tacccttgga tcgaagtaaa 9181 tttaaaactg atcagataag ttcaatgata tccagtgcag taaaaaaaaa aaatgttttt 9241 tttatctact ttccgcaaaa atgggtttta ttaacttaca tacatactag gcgcgccgaa 9301 gcatatgttc ggcttgtcga catgcccgcc gtgaccgtcg agaacccgct gacgctgccc 9361 cgcgtatccg cacccgccga cgccgtcgca cgtcccgtgc tcaccgtgac caccgcgccc 9421 agcggtttcg agggcgaggg cttcccggtg cgccgcgcgt tcgccgggat caactaccgc 9481 cacctcgacc cgttcatcat gatggacca //