Lineage and Clonal Analysis

Brief descriptions and links to pages for lineage and clonal analysis system stocks available at the BDSC are shown here (in alphabetical order). The pictures are examples and may not represent all that is available. See relevant pages for all the possibilities for each system.

BrainbowG-TRACE and i-TRACETie-Dye
CoinFLPLineage filteringTwin spot MARCM
DFS (female germline)photoreceptor clones 
FlyBowRaeppli 
Stochastic cre-mediated flip-outs between matched lox sites result in clones marked with one of several possible marker combinations.
       
 
FLP-out of one of two overlapping FRT or FRT3 stop cassettes results in GAL4- or lexA-expressing clones.
        CoinFLP
 
Stocks for assessing gene function in the female germ line by clonal analysis using "ovo[D]" stocks.
DFS
 
Stochastic FLP-mediated inversions and/or FLP-outs result in clones marked with one of several possible marker combinations.
        flybow
 
G-TRACE (GAL4 Technique for Real-time And Clonal Expression)
Stochastic FLP-out of a stop cassette marks a clone with a fluor.
G-TRACE
 
FLP-out cassettes are used to restrict ('filter') expression to the descendents of specific cells.
Lineage filtering
 
FLP-out of a stop cassette results in phiC31 integrase expression. Subsequent stochastic attP-based rearrangements result in expression of fluors under UAS or lexA control. Resulting clones can be marked with one of ten possible color combinations.
        Raeppli
 
Stochastic FLP-out of a stop cassette from three constructs results in clones marked with one of seven possible marker combinations plus/minus a gene of your choice.
        Tie-DYE
 
FLP-mediated recombination results in photoreceptor clones marked with one of two fluors.
        Tomato/GFP-FLP/FRT
 
Mitotic recombination separates a fluor from the RNAi that targets it, resulting in clonal expression.
        twin_spot
 

Related links   Fluors   FRT   FLP