Translocations in Bloomington stocks

Reciprocal translocations are generated when breaks occur in different chromosomes and the ends are swapped.

Translocation

A translocation is a pair of chromosomes. Each individual chromosome is called a translocation segregant and is denoted by the telomeres present.

  • Translocations that cause mutations

    Translocation breakpoints often disrupt genes. About a third of our stocks with translocations are maintained for their breakpoint-induced mutations. They are shown here.

  • Translocations associated with position-effect variegation

    The following stocks carry translocations that juxtapose euchromatic and heterochromatic regions and lead to position-effect variegation of the genes noted.

  • Translocations between balancers

    Reciprocal translocations between two balancer chromosomes assure that the balancers are always inherited together, because alternate segregation in meiosis I produces euploid progeny that live while adjacent segregation produces aneuploidy progeny that die.

    Euploid and Aneuploid gametes

    If one uses a translocation between second and third chromosome balancers to create a stock for second and third chromosome mutations, then the mutations will always be inherited together. This can be quite handy for many kinds of experiments.

    We have stocks with the following translocations between balancers:

    T(2;3)B3, CyO: TM6B, Tb[1]

    T(2;3)B3, CyO: TM6B, Tb[+]

    T(2;3)CyO-TM3, P{GAL4-Hsp70.PB}TR1, P{UAS-GFP.Y}TR1: P{GAL4-Hsp70.PB}TR2, P{UAS-GFP.Y}TR2, y[+] Ser[1]

    T(2;3)CyO-TM6

    T(2;3)CyO-TM8, CyO, Adh[nB]: TM8, l(3)DTS4[1]

    T(2;3)CyO-TM9, CyO: TM9, l(3)DTS4[1]

    T(2;3)SM6b-TM6B, ap[RK] Tb[1]

    T(2;3)SM6a-TM6B, Tb[1]

    T(2;3)TSTL, CyO: TM6B, Tb[1]

    T(2;3)TSTL14, SM5: TM6B, Tb[1]

    A full list of stocks with translocations between balancers is given here.

  • T(2;3)ap[Xa]

    T(2;3)ap[Xa] was generated between progenitor chromosomes with inversions, so the resulting chromosomes have several breakpoints which effectively balance regions of 2R and 3R:

    2Lt - 41F | 89E8 - 89C3 | 96A19 - 3Rt ; 2Rt - 58B1 | 42A3 - 58A4 | 42A2 - 41F | 89F1 - 96A18 | 89C2 - 3Lt

    Figure of T(2;3)ap[Xa] pairing with normal chromosomes at meiosis I from The genetics of the Xasta mutant in Drosophila melanogaster, Hetherington et al. (1968) Genet. Res. 12:285-294.

    The dominant Xasta mutation in the apterous gene caused by the translocation provides a convenient wing phenotype for following T(2;3)ap[Xa] in crosses. Like the translocations between balancers discussed above, second and third chromosome mutations maintained in stock with T(2;3)ap[Xa] will always be inherited together.  

    A list of T(2;3)ap[Xa] stocks is provided here.

  • Translocations for inheriting balancers by males

    T(Y;2)CyO and T(Y;3)TMS are reciprocal translocations between balancer chromosomes and the Y chromosome. Males carrying these chromosomes will transmit the balancers to only male progeny, because the translocated Y and autosome will segregate from the normal X and autosome in meiosis I. This can be useful in experimental crosses.

    T(Y;3)TMS carries P{Delta2-3}, a source of P transposase. It is useful for screens where one wishes to introduce a genomic source of transposase in one generation and assure it is absent in subsequent generations.

    T(Y;2)CyO carries a dominant temperature-sensitive lethal mutation, l(2)DTS513[1], which can be used to kill males inheriting the translocation upon heat treatment and produce only virgin females from crosses.

  • T(1;2)OR64 and T(1;3)OR60

    T(1;2)OR64 and T(1;3)OR60 are translocations associated with dominant male lethality. They are useful for maintaining stocks of dominant female sterile mutations.

  • Translocations for generating chromosomal deletions and intrachromosomal duplications

    Historically, a major use of reciprocal translocations between the Y chromosome and another chromosome was generating chromosomal deletions and duplications by combining translocation segregants from different translocations.

    Third chromosome deletion / duplication

    T(1;Y), T(Y;2) and T(Y;3) stocks for this “segmental aneuploidy” approach are available from Kyoto Drosophila Genomics and Genetic Resources for making X, second and third chromosome deletions and duplications.This approach can also be used with T(1;Y) chromosomes with breakpoints in X heterochromatin to create deletions and duplications of Y chromosome segments (see Gatti and Pimpinelli (1983)). It has proved to be the most effective way to create deletions of Y-linked male fertility genes, since conventional deletions on the Y chromosome are unstable. Translocation segregants from the following T(1;Y) stocks can be combined to make deletions.

    Note that several T(Y;2) and T(Y;3) chromosomes are maintained because their breakpoints disrupt specific Y-linked male fertility genes (see Translocations that cause mutations above). These chromosomes are not maintained for creating deletions.

  • T(1;Y)N12 and T(1;Y)R12

    T(1;Y)N12 and T(1;Y)R12 have one breakpoint in X centric heterochromatin and one breakpoint near the tip of a special Y chromosome that has y[+] marking the tip of the short arm and B[S] marking the tip of the long arm.

    Dispensable chromosone

    The B[S]-marked translocation segregant has all the Y-linked male fertility genes and all the X-linked euchromatic genes. This makes it an attached-XY [C(1;Y)] chromosome. The y[+]-marked translocation segregant is a tiny, dispensable chromosome.

    The large translocation segregants from T(1;Y)N12 and T(1;Y)R12 are the only simple C(1;Y) chromosomes in the Bloomington collection with B[S] marking the Y tip. C(1;Y)N12, the attached-XY segregant from T(1;Y)N12, was used in constructing Dp(1;Y)BSC chromosomes. As far as we know, the attached-XY derived from T(1;Y)R12 has never been used for further chromosome constructions.

  • A translocation for making a fourth chromosome terminal deletion

    T(1;4)sc[H] is a reciprocal translocation with breakpoints near the tips of the X and fourth chromosomes.

    Ts(1Rt;4Rt)sc h

    It is maintained in stock with a y[1]-marked attached-X. Males in the stock must carry both translocation segregants (Ts(1Lt;4Lt)sc[H] and Ts(1Rt;4Rt)sc[H]). Females may carry two normal chromosomes (and be phenotypically y[-]) or they may be heterozygous for Ts(1Lt;4Lt)sc[H], the fourth chromosome lacking its distal tip (and be phenotypically y[+]).

    text

    One can use the y[+]-marked translocation segregant (Ts(1Lt;4Lt)sc[H]) to deletion map mutations to the distal tip of the fourth chromosome. At least five lethal complementation groups including Cadps are removed by this terminal deletion.

    According to Sousa-Neves (2000), there are genes missing between the proximal portion of the fourth chromosome on one translocation segregant (Ts(1Lt;4Lt)sc[H]) and the distal portion of the fourth chromosome on the other translocation segregant (Ts(1Rt;4Rt)sc[H]). Consequently, T(1;4)sc[H] can be used experimentally in two ways: as the source of a terminal deletion as described above or as the source of a smaller interstitial deletion (using both translocation segregants).

    The T(1;4)sc[H] segregant carrying the tip of the fourth chromosome (Ts(1Rt;4Rt)sc[H]) can be used as a deletion of distal X genes or as an interchromosomal duplication of the distal fourth chromosome, but these experimental uses are less common. 123

  • A translocation for deleting or duplicating the 3R tip

    T(Y;3)L129 is a reciprocal translocation with breakpoints in a y[+]-marked Y chromosome and at 100C on 3R.

    Formation of T(Y:3)L129

    The y[+]-marked translocation segregant can be used as a deletion of 100C to 3Rt. This deletion removes RpL6, so heterozygotes will show Minute phenotypes and will likely have reduced viability. See Kongsuwan et al. (1986) for more details.

    The other translocation component can be used as a Y-linked duplication of 100C to 3Rt.

  • A translocation marking 2R for clonal analysis

    T(1;2)sc[S2] is a reciprocal translocation between the tips of the X chromosome and 2R. It marks the tip of 2R with y[+]. This is useful for detecting mitotic clones formed by somatic recombination events within 2R.

    Unlike similar duplications of y[+] onto the tips of other chromosome arms, both chromosomes of the reciprocal translocation T(1;2)sc[S2] must be present for flies to survive. This complicates clonal analysis crosses and we suggest you review previous studies that have used it before undertaking experiments.

  • Incidental translocations

    We have a few stocks where a translocation is present, but it has nothing to do with the primary component of the stock and serves no particular purpose.


Related links   Aberration   Duplication   Deficiency   Insertion