Dominant sterile and dominant lethal mutations

A mutation that causes dominant sterility in females (Fs) can be maintained in stock with a mutation that causes dominant sterility in males (Ms).

dominant sterile fig

Stocks with mutations that are lethal to only one sex may be maintained similarly. The T(1;2)OR64 and T(1;3)OR60 translocations are associated with dominant male lethality and are particularly useful for maintaining dominant female sterile mutations in stock.

The following list contains stocks with mutations or transgenes associated with dominant sterility or lethality.

Stock #female sterilemale sterilemale lethalChrGenotype
91242T(1;2)OR641;2y[1] w[*]; TI{GFP[3xP3.cLa]=CRIMIC.TG4.0}piwi[CR00690-TG4.0]/T(1;2)OR64, y[1] w[*]/CyO
3515Fs(2)M[1]T(1;2)OR641;2Fs(2)M[1]/T(1;2)Bld, In(2R)Cy, Bld[1]/T(1;2)OR64
3336Fs(2)X10[1]T(1;2)OR641;2Fs(2)X10[1], Adc[b-1] pr[1]/T(1;2)OR64/CyO
4434P{ovoD1-18}2RT(1;2)OR641;2P{w[+mW.hs]=FRT(w[hs])}G13 P{w[+mC]=ovoD1-18}2R/T(1;2)OR64/CyO
94203P{ovoD1-18}2RT(1;2)OR641;2y[1] w[*], P{ry[+t7.2]=hsFLP}1; P{ry[+t7.2]=neoFRT}42D P{w[+mC]=ovoD1-18}2R/T(1;2)OR64, y[1] w[*]/CyO
44212alphaTub67C[kav-18c]T(1;3)OR601;3alphaTub67C[kav-18c]/TM3, Sb[1] Ser[1]/T(1;3)OR60
42211Dhc64C[Lab-1]T(1;3)OR601;3mwh[1] Dhc64C[Lab-1] e[1]/TM3, Sb[1] Ser[1]/T(1;3)OR60
67749dsx[D]betaTub85D[D]1;3w[*]; st[1] betaTub85D[D] ss[1] e[s]/dsx[D] Sb[1] e[1]/TM6B, P{w[+mW.hs]=Ubi-GFP.S65T}PAD2, Tb[1]
42210Fs(3)Apc[1]T(1;3)OR601;3Fs(3)Apc[1] mwh[1] e[1]/TM3, Sb[1] Ser[1]/T(1;3)OR60
2149P{ovoD1-18}3RbetaTub85D[D]1;3w[*]; P{ry[+t7.2]=neoFRT}82B P{w[+mC]=ovoD1-18}3R/st[1] betaTub85D[D] ss[1] e[s]/TM3, Sb[1]
66713TI{TI}fru[Deltatra]T(1;3)OR601;3P{ry[+t7.2]=neoFRT}82B dsx[1] TI{TI}fru[Deltatra]/TM2/T(1;3)OR60
66874TI{TI}fru[M]T(1;3)OR601;3TI{TI}fru[M]/T(1;3)OR60/TM3, Sb[1] Ser[1]
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Dominant female sterile mutations or transgenes on the X chromosome are typically maintained in attached-X stocks as shown in the following table.

Stock #female sterileChrGenotype
1309ovo[D1]1ovo[D1] v[24]/C(1)DX, y[1] w[1] f[1]
1813ovo[D1]1;2w[*] ovo[D1] v[24] P{w[+mW.hs]=FRT(w[hs])}101/C(1)DX, y[1] f[1]; P{ry[+t7.2]=hsFLP}38
1318ovo[D2]1ovo[D2] v[24]/C(1)DX, y[1] f[1]
1326ovo[D3]1ovo[D3] v[24]/C(1)DX, y[1] f[1]
23880P{ovoD1-18}P4.11P{w[+mC]=ovoD1-18}P4.1, P{ry[+t7.2]=hsFLP}12, y[1] w[1118] sn[3] P{ry[+t7.2]=neoFRT}19A/C(1)DX, y[1] f[1]
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