Mitotic recombination stocks

These stocks can be used in experiments to monitor induced or spontaneous mitotic recombination. They contain mutations or chromosomal duplications that change the color or morphology of cuticular structures derived from imaginal disks.

As shown in the figure, mitotic clones can be detected when recessive marker mutations are made homozygous by somatic recombination.

mit_rec_1

Both twin spots can be revealed by marking a chromosome with a recessive marker and a transgene/ duplicated gene as shown below.

mit_rec_2

Note that both chromosomes of the reciprocal translocationT(1;2)sc[S2] must be present for flies to survive. We suggest you review previous experiments that have used it before undertaking experiments.

Some stocks have markers for assaying recombination on multiple arms simultaneously.