SuRe Recombinator target sites

For a brief explanation of the SuRe system, first see our SuRe-CR page. For more information on how target sites are defined, stay here . . .

For SuRe to work, the Adaptors, AD1 and AD2, need to be on the opposite 'ends' of the two regions you are trying to combine. The Recombinator lines facilitate this by using gRNAs and homology regions to target appropriate regions in integrated transgenes based on the position of the region in the original vector prior to integration.

  • The R1 Recombinator facilitates insertion of Adaptor 1 (AD1) upstream of the vector's attB site. When integrated into a landing site, this region lies between the transgene and attL.
  • The R2 Recombinator facilitates insertion of Adaptor 2 (AD2) downstream of the vector's attB site. When integrated into a landing site, this region lies between the transgene and attR.

 schematic of attB vector recombination into an attP landing site

SuRe uses the standard attB sequences provided by FlyBase (see links below) to define 'upstream' and 'downstream' in the original vector:

To determine if your transgene of interest will work with currently available adaptors please see sure_adaptors.xlsx (or sure_adaptors.csv), and our sure sequences page for info on the gRNA sequences and homology regions carried in each R strain.