Split-GAL4 or split-lexA combination lines

The tables below contain stocks carrying both "halves" of a split-GAL4 combination, ie., they carry both a DNA-binding domain (DBD) and activation domain (AD) hemidriver. The majority come from the Janelia FlyLight Project which generated a set of stocks carrying combinations of split transcriptional activators that activate UAS reporters in specific sets of neurons in the adult mushroom body and optic lobe. Another set of Janelia-based stocks has been characterized by Marta Zlatic's group for expression in larval neural circuits.

The expression patterns for FlyLight-characterized lines can be found at the Janelia FlyLight site and the Virtual Fly Brain (VFB) site. Links to the data for individual lines are provided in the FlyLight and VFB columns. See the Janelia FlyLight site and Meissner et al. (2023) A split-GAL4 driver line resource for Drosophila CNS cell types for more information and references.

Be aware that preliminary data from the BDSC suggests that about 40% of these split combination lines show additional expression outside their described nervous system expression. We strongly suggest examining whole larvae and adults for expression beyond what has been published, especially if expression outside the characterized pattern could impact your analyses.

Control stocks - These stocks carry the GAL4 DNA binding domain and/or the p65 activation domain in the same vector used to make the Janelia split GAL4 collection but with no regulatory fragment present.

Stk #GenotypeAlso known as
71209w[1118]; P{GAL4-DBD.Uw}attP2'GAL4-DBD.empty' control, BPZpGDBD or pBPZpGAL4DBDUw (attP2).
71210w[1118]; P{p65-AD.Uw}attP40'p65-AD.empty' control, BPp65ADZp or pBPp65ADZpUw (attP40).
79603w[1118]; P{p65-AD.Uw}attP40; P{GAL4-DBD.Uw}attP2'empty stable split' control

For other stocks expressing both an activation and DNA-binding domain see:
- Transcription-based calcium sensors

For stocks expressing either an activation OR a DNA-binding domain see:
- Split transcriptional activator page

The majority of the stocks in the first section below were generated by Janelia and are available as a predefined set for ordering. The second section contains stocks carrying one or more split combos in the same attP site. They were generated by the Schnitzer lab via the SuRe system.