Duplication Lists and Uses

BDSC carries two sets of duplications: Interchromosomal Duplications and Intrachromosomal Duplications as described below:

Interchromosomal Duplications
We carry molecularly-defined duplications that provide nearly complete coverage for X chromosome and 4th chromosome. However only a few molecularly-defined duplications for Chr 2 and Chr 3 are currently available. BDSC also has a large collection of cytologically-defined duplications generated by irradiation and characterized primarily by polytene chromosome cytology.

  • Y-linked X Chr duplications - The duplicated X chromosome segments in this set range from more than 160 genes down to only a few genes and they are arranged in nested sets. The breakpoints provide mapping resolution of a median of nine genes. This is the most convenient set to use for duplication mapping X-linked mutations.
  • Transgenic X Chr duplications - The duplicated segments in this set were generated by transgenic methods and contain around 5 to 20 genes. Most are inserted on the third chromosome. This set may provide higher mapping resolution in some regions and may be more convenient for duplicating particular genes.
  • X Chromosome Duplication Kit - To simplify the initial mapping of X-linked mutations, we have assembled this kit to provide complete coverage with the fewest molecularly defined duplications from the sets above.
  • Autosomal duplications - We have few molecularly-defined duplications for Chr 2 and Chr 3 but duplications available for Chr 4 provide nearly complete fourth chromosome coverage with transgenic segments carried on the third chromosome.

Please see An Introduction to Interchromosomal Duplications for more information about these cytologically-defined Dps.

  • X Chr - These are older duplications generated by irradiation and characterized primarily by polytene chromosome cytology. They vary in size and insertion site, but most are much larger than transgenic duplications found above. Typically, these duplications are now used to supplement the sets above or when one needs a duplicated segment inserted at an alternative site.
  • Chr 2, Chr 3, Chr 4 - Most autosomal duplications at Bloomington were generated by irradiation and are characterized primarily by polytene chromosome cytology. They vary in size and insertion site.
Intrachromosomal Duplications

We list intrachromosomal duplications separately because they are primarily used for changing the dosages of genes and are less useful for gene mapping and rescue experiments. All have been characterized by polytene cytology. You can read more about these duplications in An Introduction to Intrachromosomal Duplications

Follow the links below for all stocks carrying intrachromosomal duplications and subsets that provide efficient coverage of the indicated chromosome:

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