The UAS constructs in the stocks below express proteins or protein domains tagged with miniSOG, an enzymatic tag that converts diaminobenzidine to an osmophilic substrate. Using these stocks, miniSOG can be directed to the cytoplasm, cell membrane, actin fibers, mitochondria or synapses. Some of the miniSOG-tagged proteins are also tagged for epifluorescence microscopy.
The technique was described in Ng et al. (2016) “Genetically targeted 3D visualisation of Drosophila neurons under Electron Microscopy and X-Ray Microscopy using miniSOG” Scientific Reports 6: 38863.