phiC31 Stocks

Traditionally, transgenic flies are created based on P-element mediated transformation, which often resuts in random insertions in the genome. There is also some positional effect on transgene's expression. More recently, phiC31 (ΦC31) integrase system has been used to create site-specific integration. The bacteriophage phiC31 integrase encodes a serine-type recombinase that mediates the sequence-specific recombination between two attachment sites, attB and attP. Because the two sites recognized by the phiC31 integrase differ and the recombination process mixes the two sites into two different sites (attR and attL), phiC31 based integration is unidirectional. For more information about the phiC31 system, see FlyC31.

phiC31 integrase stocks
phiC31 attP stocks
phiC31 stocks for RMCE

Primary references:

P{CaryP} insertions: Groth et al. 2004  and Szabad et al. 2012

PBac{y[+]-attP} insertions:  Venken et al. 2006

M{3xP3-RFP.attP}, M{vas-int.B} and P{nos-phiC31\int.NLS} insertions: Bischof et al. 2007 (see FlyC31 for additional information the phiC31 integrase system)

M{vas-int.Dm} insertions: K. Basler and F. Karch, FlyC31

P{attP.w[+].attP} insertions for Recombinase-Mediated Cassette Exchange (RMCE): Bateman & Wu, 2008, Sun et al., 2012

Position effects of attP sites on transgene expression: Markstein et al. 2008, Nature Genetics 40, 476 - 483.