Chemoconnectome (CCT) is defined as the entire set of neurotransmitters, neuromodulators, neuropeptides, and their receptors underlying chemotransmission in an animal. The Rao lab has generated knockout lines of CCT genes for functional investigations and knockin lines containing Gal4 and other tools for examining gene expression and manipulating neuronal activities, with a versatile platform allowing genetic intersections and logic gates.
Methods are described in Deng et al. 2019. Chemoconnectomics: Mapping Chemical Transmission in Drosophila. Neuron 101(5): 876--893.e4.