UAS constructs for mosquito odorant receptors

BDSC carries two sets of stocks that allow mosquito odorant receptors to be expressed in Drosophila under UAS control.

The UAS constructs are maintained with the aberrations Df(2L)dp-79b and Dp(2;2)dpp[d21], which together create a synthetic deletion for the Or22a and Or22b genes (commonly called “Delta halo” flies). Antennal olfactory receptor neuron ab3A loses its odorant response in flies lacking these genes. This “empty neuron” system (Dobritsa et al., 2003) allows the function of any odorant receptor to be tested in the absence of the naturally occurring odorant receptors.

  • Aedes aegypti odorant receptors

    In the first set of stocks, variants of Odorant receptor 4 from Aedes aegypti are expressed under UAS control. Mosquitoes differ in their preference for humans over other animals depending on the Or4 alleles they carry.

    These stocks were described in McBride et al. (2014): Evolution of mosquito preference for humans linked to an odorant receptor, Nature 515: 222–227.

  • Anopheles gambiae odorant receptors

    The second set of stocks carry UAS constructs for Anopheles gambiae odorant receptors. The stocks were described in Carey et al. (2010): Odorant reception in the malaria mosquito Anopheles gambiae, Nature 464: 66–71.

Related link   UAS   GAL4