The following stocks were used in a lesson plan published by Derek Dean, David Deitcher, David Loehlin, and Lois Banta (Dean et al., 2020. Mapping a mutation to its gene: The "fly lab” as a modern research experience.CourseSource). The lesson simulates a modern research experience: students cross an unknown mutant to flies with molecularly-defined transgene insertions, deletions, and duplications. After scoring the progeny, they interpret their data with FlyBase bioinformatics to map the unknown mutation to a single gene.

If you do not wish to order the complete list of stocks, below the table are suggestions about which stocks to prioritize.

Complete list of stocks to order:

Here is a complete list of the stock numbers for easy copy/paste into the BDSC order form: 7718, 8898, 8952, 9058, 12442, 12470, 26565, 26863, 26864, 30380, 30381, 30382, 30383, 30384, 30385, 30386, 30387, 30389, 30390, 32580, 32628, 33328, 84699

If you prefer to work with fewer stocks, we recommend the following priorities (reasoning discussed in Supporting File S1 of the manuscript, Table S1-4):

Parental Stocks

• 84699 is essential to order (it is the "Mutant" stock)
• Order at least three "WT" stocks:
  • at least one of the following "WT" stocks: 12442, 12470, 33328
  • at least one of the remaining "WT" stocks: 32580, 32628
  • at least one more "WT" stock (any other from the 5 listed in the previous two bullet points)

Df Stocks

• 7718
• at least one of the following: 8898, 8952, 9058

Dp Stocks

• 30384
• 30385
• at least one other Dp stock from the remaining list: 30380, 30381, 30382, 30383, 30386, 30387, 30389, 30390