Imprecise Excision of Transposons

When P or Minos element insertions are exposed to transposase, they can excise imprecisely to create chromosomal deletions. The frequencies of imprecise excisions and the sizes of deletions made by imprecise excisions are larger if insertions are exposed to transposase in a DNA repair-defective genetic background with a Blm mutation. The following stocks are useful for such screens.

For stocks carrying various transposases, see the Tranposable Element Mutagenesis page.

Stock #GenotypeChromosome
8656Blm[D3] ry[506]/TM3, Sb[1] ry[RK]3
8657st[1] Blm[D2] Sb[1] P{ry[+t7.2]=Delta2-3}99B/TM6B, Tb[1]3
24613w[1118]; sna[Sco]/SM6a, P{hsILMiT}2.41;2
28878w[1118]; Blm[N1]/TM3, Sb[1]1;3
28879y[1]/Dp(1;Y)y[+]; Blm[N2] ry[506] Sb[1] P{ry[+t7.2]=Delta2-3}99B/TM6B, Tb[1]1;Y;3
36311y[1] w[*]; sna[Sco]/SM6a, P{hsILMiT}2.41;2

  • Witsell et al., 2010. Super-sized deletions: Improved transposon excision screens using a mus309 mutant background. Fly 4: 137-140.
  • Witsell et al., 2009. Removal of the bloom syndrome DNA helicase extends the utility of imprecise transposon excision for making null mutations in Drosophila. Genetics 183: 1187-1193.