Auxin-inducible protein degradation

The TIR1 protein from rice (Oryza sativa) binds to proteins with an auxin-inducible degron domain (AID) in the presence of auxin to direct proteasomal degradation. P{Ubi-OrTIR1.T} expresses TIR1 ubiquitously under the control of Ubi-p63E regulatory sequences. Proteins tagged with an AID will be degraded when flies are fed auxin. This system was described in Trost et al. (2016) “Regulated protein depletion by the auxin-inducible degradation system in Drosophila melanogaster” Fly 10: 35–46.


Similarly, P{UAS-OsTIR1.myc} and P{UASp-OrTIR1.myc} express TIR1 under UAS control to eliminate AID-tagged proteins in the cell-specific patterns determined by GAL4 drivers. See Bence et al. (2017) “Combining the auxin-inducible degradation system with CRISPR/Cas9-based genome editing for the conditional depletion of endogenous Drosophila melanogaster proteins” FEBS J. 284: 1056–1069.


TI{TI}vas[AID:EGFP] expresses EGFP-tagged vasa protein with an AID tag that can be eliminated upon auxin treatment.


PBac{tubP-TIR1-T2A-GAL80.AID} expresses both AID-tagged GAL80 and TIR1 ubiquitously under the control of alphaTub84B regulatory sequences. It allows GAL4-induced expression of UAS constructs to be repressed by GAL80 until flies are fed auxin.

Related links   ubiquitination tag