Photoactivatable adenylyl and guanylyl cyclases

P{UAS-bPAC.S} encodes a bacterial adenylyl cylase that converts adenosine triphosphate to cyclic adenosine monophosphate upon exposure to blue light. It was described in Stierl et al. (2011) “Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa” J. Biol. Chem. 286: 1181–1188.

and is present in the following stocks:

M{UAS-bPAC.M} is similar to P{UAS-bPAC.S}. M{UAS-bPGC.M} is identical to M{UAS-bPAC.M} except the adenylyl cyclase sequence was modified, as described in Ryu et al. (2010) “Natural and Engineered Photoactivated Nucleotidyl Cyclases for Optogenetic Applications” Journal of Biological Chemistry 285: 41501–4150, to produce an enzyme with high guanylyl cyclase activity and low adenylyl cyclase activity. Specifically, the enzyme has K197E, D265K and T267G amino acid substitutions.

The constructs are present in the following stocks:

As of August 2021, neither stock had been rigorously tested for adenylyl and/or guanylyl cyclase activity in crosses to GAL4 driver stocks.