TRiP Toolbox Stocks for CRISPR Cas9

TRiP Functional Genomics Resources is producing guide RNA transgenes for both overexpression and knockout of genes via CRISPR/Cas9 mechanisms. The stocks listed below were made by TRiP to facilitate use of these stocks.

P{UAS-3xFLAG.dCas9.VPR} expresses a nuclease-dead Cas9 fused to a chimeric activation domain under the control of UAS. It can be used to activate transcription of a gene in the presence of a guide RNA designed to facilitate overexpression of that gene. For more details see the TRiP-CRISPR overexpression site. The stocks below pair UAS-Cas9.VPR with various GAL4 and/or GAL80 insertions.

P{UAS-Cas9.P2} expresses Cas9 under the control of UAS. It can be used to create small insertions or deletions in a gene in a tissue-specific manner or in the germline in the presence of a guide RNA. For more details see the TRiP-CRISPR knockout site. The stocks below pair UAS-Cas9 with various GAL4 and/or GAL80 insertions.