Guide RNA Stocks

All available CRISPR guide RNA lines are listed here. The insertions must be combined with a source of Cas9 to induce gene knockout or overexpression. See our Cas9 page for available Cas9 stocks and the TRiP-CRISPR toolbox page for a list of available stocks with UAS-Cas9 and a GAL4 driver.

The vast majority of lines were made by the Transgenic RNAi Project and are divided into three collections (for specifics, please see the TRiP in vivo CRISPR site):

  1. flySAM2.0 - Expresses sgRNA ubiquitously plus Cas9 and MCP::p65(h)::HSF1 under the control of UAS for multiplex CRISPR-based gene activation. Activate expression of the target gene in somatic and/or germline cells by crossing to a line expressing GAL4. 
  2. TRiP-CRISPR Overexpression (TRiP-OE) - expresses two sgRNAs ubiquitously (Cas9 is not included).  Activate expression of the target gene in somatic and/or germline cells by crossing to a line expressing GAL4 and dCas9-VPR.
  3. TRiP-CRISPR KnockOut (TRiP-KO) - expresses sgRNA ubiquitously (Cas9 is not included).  Generate indels (small insertion or deletion mutations) in somatic and/or germline cells by crossing to a line expressing GAL4 and Cas9.

The flySAM2.0 lines are generated in the flySAM2.0 vector (Jia et al., 2018). For details see the TRiP CRISPR vector page. TRiP-TKO and TRiP-OE lines are generated in the pCFD3 or pCFD4 vector, respectively (Port et al., 2014). Detailed descriptions of these vectors are available at the CRISPR Fly Design site.

The TRiP encourages the community to nominate genes for sgRNA production via the TRiP sgRNA Database. If use of any of the TRiP-CRISPR stocks results in data used in a publication, please cite the TRiP in your publication.

** Some guide RNAs target more than one gene. In the tables below, the "# Targets" column indicates the number of targets per transgene. To see the other targets, either filter the table with the stock number or click on the stock number to read the stock report.