All available CRISPR guide RNA lines are listed here. The insertions must be combined with a source of Cas9 to induce gene knockout or overexpression. See our Cas9 page for available Cas9 stocks and the TRiP-CRISPR toolbox page for a list of available stocks with UAS-Cas9 and a GAL4 driver. For a nice review of CRISPR technology with helpful definitions and schematics, see Zirin et al., 2022.

These lines are divided into two types - 1) gRNAs designed to work with a specialized Cas9 to activate target gene expression and 2) gRNAs designed to work with Cas9 to generate indels in the target gene. Available stocks are divided into the following categories:

gRNAs for expression
These lines were generated by the Transgenic RNAi Project for overexpression of genes (TRiP-OE or TOE lines). They express two sgRNAs ubiquitously (Cas9 is not included). Activate expression of the target gene in somatic and/or germline cells by crossing to a line expressing GAL4 and dCas9-VPR.

gRNAs for expression - flySAM2.0
These lines were generated by the Transgenic RNAi Project for overexpression of genes (Sam.dCas9 lines). They express sgRNA ubiquitously plus Cas9 and MCP::p65(h)::HSF1 under the control of UAS for multiplex CRISPR-based gene activation. Activate expression of the target gene in somatic and/or germline cells by crossing to a line expressing GAL4.

gRNAs for knock out
The majority of these lines were generated by the Transgenic RNAi Project (TRiP-KO or TKO lines) or the Weizmann Knock Out project (WKO lines). They express sgRNA ubiquitously (Cas9 is not included). Generate indels (small insertion or deletion mutations) in somatic and/or germline cells by crossing to a line expressing GAL4 and Cas9.

The flySAM2.0 lines are generated in the flySAM2.0 vector (Jia et al., 2018). TRiP-TKO, TRiP-OE and WKO lines are generated in pCFD3 or pCFD4 or pCFD5 vectors (Port et al., 2014). Detailed descriptions of these vectors are available at the CRISPR Fly Design site.

For more details on TRiP stocks, please see the TRiP in vivo CRISPR site. The TRiP encourages the community to nominate genes for sgRNA production via the TRiP sgRNA Database.

For more details on the WKO stocks, please see Meltzer et al., 2019.

** Some guide RNAs target more than one gene. In the tables below, the "# Targets" column indicates the number of targets per transgene. To see the other targets, either filter the table with the stock number or click on the stock number to read the stock report.