If you wish to import nontransgenic strains of any Drosophila species, you must obtain a permit from a division of the USDA called Plant Protection and Quarantine (PPQ). Applications are submitted using the e-permits system. To use the e-permits system, you must first register online.
A PPQ import permit may be used for multiple shipments in a three-year period from any of the countries or continents you designate in your application. See What should I do with permits once I receive them?
If you have already received an import permit and it has expired, you can renew the permit online.
If you have questions about importing nontransgenic strains, email Plant Protection and Quarantine or call 877-770-5990.