Minos Transposable Element Stocks

Lists of Minos-based insertions selected by the Gene Disruption Project (GDP) can be found under the links below. For most insertions mapped to the sequence, the cytology is estimated using the FlyBase Cytological<==>Sequence map conversion table.

Minos Insertions (click a chromosome number below for a list of stocks)
Chromosome1234

Please read this caution about insertion phenotype data.

Stock carrying Minos transposase
24613  w[1118]; noc[Sco]/SM6a, P{hsILMiT}2.4   
General information

- Schematics for these insertions can be found at the  GDP website.

- Markers:

Mi{ET1} - EGFP is the only scoreable marker carried by  Mi{ET1}. The construct is not marked with white, yellow, rosy or any other visible marker.

Mi{MIC} -  Mi{MIC} is marked with  y[+mDint2].

- Background:
The  Mi{ET1} insertions were generated in the same genetic background as the  DrosDel Project insertions and deletions.  DrosDel starter stocks can be used to manipulate these Minos stocks while retaining the isogenic background. The  Mi{MIC} insertions are in a different (undefined) background.

The Mi{MIC} element contains a mutagenic gene trap cassette that consists of a splice acceptor site, stop codons in all three reading frame, the coding sequence of EGFP and an SV40 polyadenylation signal. A y[+mDint2] marker is present downstream of this sequence. The gene trap cassette and the y[+mDint2] marker are flanked by inverted attP sites, which allows for the replacement of this entire sequence with DNA from a compatible donor plasmid (where the sequence to be inserted is flanked by inverted attB sites) through recombination-mediated cassette exchange (RMCE) driven by the phiC31:int integrase (Venken et al., 2011, Nagarkar-Jaiswal et al., 2015). 

Note that the EGFP sequence present in the Mi{MIC} element does not play a role in its mutagenic activity and is typically not expressed in Mi{MIC} insertions that are in coding introns. However, a cryptic ribosomal initiation site is present before the EGFP sequence, and thus if a Mi{MIC} element is inserted in the 5'UTR of a gene, it can act as a gene trap, resulting in EGFP expression in these cases (Kanca et al., 2017).

Inverse PCR protocols and primers are available from the  Gene Disruption Project web site.

Primary references:

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