This collection of X chromosome lethals was generated by Hugo Bellen's lab as part of a forward genetic screen utilizing low concentrations of EMS to minimize lethal hits per chromosome. Acquisition of this collection is ongoing. More stocks will be added as they become available.

Briefly, y[1] w[*] P{neoFRT}19A chromosomes were mutagenized and lethal mutations were isolated. Selection was based on phenotypic characterization of homozygous mutant tissues in two types of clones – electroretinograms of ey-FLP eye clones and morphological observation of wings and thoraces of Ubx-FLP clones. Complementation tests, duplication mapping and P[acman] mapping were performed, and mutations in selected cases were determined by PCR and Sanger sequencing and/or whole genome sequencing.

For more information see:

• Yamamoto et al., 2014. A drosophila genetic resource of mutants to study mechanisms underlying human genetic diseases. Cell. 2014 Sep 25;159(1):200-14. doi: 10.1016/j.cell.2014.09.002.
• Haelterman et al., 2014. Large-scale identification of chemically induced mutations in Drosophila melanogaster. Genome Res. 2014 Oct;24(10):1707-18. doi: 10.1101/gr.174615.114.

All mutations are balanced over FM7c, Kr-GAL4 UAS-GFP sn+.