Transcriptional effectors from the tet, pip, ttg and van prokaryotic operons have been adapted to activate transcription in Drosophila by fusing their DNA binding domains to the VP16 transcriptional activation domain. The tetracycline-controlled transactivator (tTA) can be inactivated by treating flies with tetracycline or doxycycline.
The following stocks carry responder transgenes with binding sites (“Operator sequences”) for transcriptional effectors from the tet, cym, pip, ttg or van operons.
Bello, B., Resendez-Perez, D., Gehring, W.J. (1998). Spatial and temporal targeting of gene expression in Drosophila by means of a tetracycline-dependent transactivator system. Development 125: 2193--2202.
Stebbins, M.J., Yin, J.C.P. (2001). Adaptable doxycycline-regulated gene expression systems for Drosophila. Gene 270: 103--111.