This recipe came from Harvard Biolabs, although they now use a dextrose medium. It has not been used at Bloomington. The cooking instructions assume that your are cooking in a steam-jacketed kettle with an automatic mixer.
Ingredients
| Water | 8.5 | liters |
| Agar | 79 | grams |
| Torumel yeast | 275 | grams |
| Cornmeal | 520 | grams |
| Granulated sugar | 1,100 | grams |
| p-Hydroxy-benzoic acid methyl ester | 23.8 | grams |
| 95% ethanol | 91.8 | milliliters |
| *adjust agar concentration according to the gel strength of your agar | ||
Instructions
- Boil water.
- Add agar, stirring constantly.
- After agar has dissolved, add yeast, cornmeal and sugar and continue stirring until mix boils again.
- Shut off steam.
- Allow the mix to cook with the mixer running for about 15 minutes.
- Dissolve p-Hydroxy-benzoic acid methyl ester in ethanol and add to medium; mix thoroughly.
- Dispense.
- Allow vials to cool for several hours (covered snugly with cheesecloth) before plugging. Allow bottles to sit overnight (covered) before plugging.