Cornmeal, Sucrose and Yeast Medium

Torumel yeast275grams
Granulated sugar1,100grams
p-Hydroxy-benzoic acid methyl ester23.8grams
95% ethanol91.8milliliters
*adjust agar concentration according to the gel strength of your agar
  1. Boil water.
  2. Add agar, stirring constantly.
  3. After agar has dissolved, add yeast, cornmeal and sugar and continue stirring until mix boils again.
  4. Shut off steam.
  5. Allow the mix to cook with the mixer running for about 15 minutes.
  6. Dissolve p-Hydroxy-benzoic acid methyl ester in ethanol and add to medium; mix thoroughly.
  7. Dispense.
  8. Allow vials to cool for several hours (covered snugly with cheesecloth) before plugging. Allow bottles to sit overnight (covered) before plugging.