This rich medium was described by Backhaus et al. (1984). Although expensive, flies are much more productive on this medium compared to cornmeal-molasses-yeast food (at least 10x more productive in our hands) and it pumps extremely well. The recipe below makes 1 liter and can be easily cooked in a microwave oven. Nutri-Fly™ "German Food" Sick Fly Formulation (Genesee Scientific) is a pre-mixed version of this recipe.
|MgSO4 x 6H2O||0.5||grams|
|CaCl2 x 2H2O||0.5||grams|
|10% p-Hydroxy-benzoic acid methyl ester in 95% ethanol||10||milliliters|
|Distilled Water||add to above to make 1 liter|
|*adjust agar concentration according to the gel strength of your agar|
- Stir, one at a time, each ingredient except propionic acid and p-hydroxy-benzoic acid methyl ester into most of the water. Add additional water to bring the total to 1 liter.
- Bring mixture to a boil and turn off heat source.
- Cool to 60oC.
- Add p-hydroxy-benzoic acid methyl ester, stirring constantly.
- Add propionic acid just before dispensing.
- Dispense when the temperature of the mixture is between 40o and 60oC. The medium will separate if it is too hot when poured.
- Store at 8oC until ready to use.