Hard Agar Substrate

Ingredients
Water2.25liters
Agar88grams
Light corn syrup350milliliters
10% p-Hydroxy-benzoic acid methyl ester in 95% ethanol38milliliters
Instructions
  1. Mix water, agar and syrup.

  2. Boil until agar is dissolved.

  3. Cool for 5 minutes or more, stir in p-Hydroxy-benzoic acid methyl ester.

  4. Dispense into petri dishes.

Notes
Agar
The amount of agar should be determined empirically for your brand and lot of agar. We buy 100 lb. barrels of "Agar 700" (product #40004) from Mooragar, Inc.
Corn Syrup
We use Karo® light corn syrup.